báo cáo hóa học: " Activation of α7 nicotinic acetylcholine receptor by nicotine selectively up-regulates cyclooxygenase-2 and prostaglandin E2 in rat microglial cultures" pdf

... microglia by promoting and/ or inhibiting the release of other important pro- inflammatory and lipid mediator such as prostaglandin E 2 . Methods: Expression of α7 nicotinic receptor in rat microglial ... [29- Western blot and fluorescent immunostaining of α7 nAChR in rat microglial culturesFigure 2 Western blot and fluorescent immunostaining of α7 nAC...

Ngày tải lên: 19/06/2014, 22:20

... circulation after infusion if cell adhesion molecules and chemokine receptors as also upregulated in vivo as part of activation. In vitro, we have observed increases in CD54 and CCR7 (10-fold and 1.4-fold increase ... use of CD40 and TLR ago- nists [26]. A more recent analysis of combined vs. mono- therapy in a mouse melanoma model showed that combined activation via...

Ngày tải lên: 18/06/2014, 15:20

... expressed iNOS produces high, sustained levels of NO which can, in certain conditions, kill nearby neurons, by mechanisms including inhibition of mitochondrial respiration and the release of glutamate ... by inhibitors of iNOS, NADPH oxidase and a peroxynitrite scavenger, but not by the NMDA receptor blocker. Activation of NADPH oxidase in the presence of iNOS ex...

Ngày tải lên: 19/06/2014, 22:20

... et al.: Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy. Journal of Neuroinflammation ... be directly involved in the formation of retinal NV in the later stages of the OIR model. In this study, we investigated the temporal and spatial distribution of micr...

Ngày tải lên: 19/06/2014, 22:20

... expression in cumulus cells cultured with FSH and/ or LH have remained unclear during in vitro mat- uration of porcine COCs. The binding of FSH and/ or LH in granulosa cells to its own receptors ... reported that during the ovulation process the PKA-dependent pathway involving the ligand activation of G protein-coupled receptors by their ligands in cumu- lus and/ or granu...

Ngày tải lên: 20/06/2014, 07:20

... intensities of both shifted bands in hA-treated cells. In addition, the increased CRE binding activity was suppressed by JNK inhibitor I and SB 203580, implying that hA-evoked CRE binding is mediated by ... caspase-3 activation by 30%; as did combined SB203580 and JNK inhibitor I, by about 70%; and the combination of SB203580, the JNK inhibitor I and a caspase-8 inh...

Ngày tải lên: 07/03/2014, 12:20

... to harvesting and counting in a liquid scintillation counter. The drugs were used in the following concentrations: vinblastine and vincristine, 1 nM; paclitaxel, azadeoxycytidine, and methotrexate, ... agent (concentration, nM) Apoptosis of DCs (% ± SEM) vinblastine (50) 3.2 ± 0.9 vinblastine (10) 1.1 ± 1.3 vinblastine (1) 0.9 ± 0.3 vinblastine(0.1) -0.6 ± 0.3 vincristine (50)...

Ngày tải lên: 18/06/2014, 15:20

... the V600E and V600K mutations. Our preclinical data demonstrating s imilar kinase activity of the V600K and V600E mutations, together with clear evidence of clinical activity of PLX4032 in a patient ... mutations should be included in current and future trials of BRAF inhibitors. Acknowledgements This work was supported by the Yale SPORE in Skin Cancer funded by the Na...

Ngày tải lên: 18/06/2014, 16:20

... NSC cultures and the CSF of the rats. The immunochemical staining for MHC in brain sections and the enzyme-linked immunosorbent assay of CSF were carried out in sham-operated rats and rats with ... staining. Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in cultures and track the migration and...

Ngày tải lên: 18/06/2014, 16:20

... that activation of TLR4 induces NADPH oxidase activation and ROS production in cardiomyocytes [15]. The activation of TLR4 and it’s down-stream signal- ing pathways lead to up-regulation of TNF and ... signaling and result in the apoptosis of cardiomyocytes. Finally, we explored the therapeutic intervention of apoptosis using siRNA. Specific silencing of genes wi...

Ngày tải lên: 18/06/2014, 16:20