... thermalcycler. After 3 min denaturation at 94°C, 30 PCR cycleswere performed, each cycle consisting of 94°C for 1.5min, 65°C for 1 min and 72°C for 1 min, and a final 6 minelongation at 72°C. ... pmol of 6FAM-TAMRA probe and PCR Bufferincluding 4.5 mM MgCl2. After the initial denaturation at95°C for 5 min, 45 cycles consisting of 95°C for 30 sec and 67°C for 1 min were performed. If ... Biological Technology Ltd, Wuhan, China), washed and incubated with 1:50dilution of fluorescein labeled goat anti-mouse Ig (BosterBiological Technology Ltd, Wuhan, China). The slideswere counterstained...