... Matrigel coating a filter using chemoinvasion chambers. Statistical analysis Data are presented as mean ± SD. All statistical analysis was performed using STATVIEW for Macintosh. The Mann– Whitney ... has been ascribed to activation of the classical pathway (Ras fi c-Raf1 fi ERK signaling cascade) [51]. An alternative pathway consists of the sequential activation of Rac1, MEKK1, c-...
Ngày tải lên: 17/03/2014, 11:20
... B.F) staining. (C) Quantita- tive analysis of GW9662 inhibition of adipocyte apoptosis. AO ⁄ EB, apoptosis measured by AO ⁄ EB staining; Hoe ⁄ B.F, apoptosis measured by Hoechst 33258 staining. ... against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GA AATCGTGCGTGACATCAAAG-3¢; actin reverse, 5¢-TG TAGTTTCATGGA TGCCACAG-3¢; Akt-1 forward, 5¢ -A A...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... growth factors. All of these transmembrane receptors contain protein tyrosine kinase activity in their intracellular domain [1]. The signalling pathway of insulin is similar to that of some other ... adhesion to laminin was increased with the concen- trations of the coated laminin, and enhanced after insulin treatment at different concentrations of laminin. By con- tra...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo y học: "Low socio-economic status, smoking, mental stress and obesity predict obstructive symptoms in women, but only smoking also predicts subsequent experience of poor health"
... predictor of asthma incidence in women but not in men. The authors speculate that female sex hormones may play an important role in the aetiology of asthma and that these hormones are influenced by ... this population, in which reduced PEF increased the risk of cardiovascular disease (CVD) and death twelve years later, independent of the presence of risk facto...
Ngày tải lên: 31/10/2012, 15:34
Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... format from the amino terminus to the carboxyl terminus, with repeats of the ectodomain shown as rectangles. The shaded rectangles indicate repeats 3 and 9, to which the main determinants of Man-6-P ... receptor is 45–70 A ˚ , indicating that a single diphosphorylated oligosaccha- ride, with a maximum distance of approximately 30 A ˚ [35], could not bind to the...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... protein, lacking the copper binding domain is cap- able of domain swapping and forms a dimer as revealed by crystal structure analysis [36]. In our case, SEC data collected for samples at pH 7 have ... versa were made in an enclosed reaction cell maintained at a constant temperature. The instrument measured the heat generated or absorbed as the ligand-macromolecule react...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: "PARALLEL MULTIPLE CONTEXT-FREE GRAMMARS, FINITE-STATE TRANSLATION SYSTEMS, AND POLYNOMIAL-TIME RECOGNIZABLE SUBCLASSES OF LEXICAL-FUNCTIONAL GRAMMARS" docx
... val- ues of lls and r/s on v are defined and equals with each other. In this case, it is also said that Fv locally satisfies the functional schemata of p. NOTE : Because the meaning of a ... be a derivation tree of a derivation A =** Go a. If all internal nodes in t satisfy functional schemata, then a is said to be derived from A and written as A =~...
Ngày tải lên: 08/03/2014, 07:20
Báo cáo khoa học: Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY are novel substrates of fibroblast activation protein-a pdf
... throughout the brain, and is involved in the regulation of energy balance by stimulating increased food intake. Pep- tide YY (PYY) is produced by the gastrointestinal tract, and, via NPY receptor binding, ... important role in maintaining cardiovascular homeostasis by acti- vating guanylyl cyclase A [47]. In addition, BNP has been shown to inhibit liver fibrosis by...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Highly site-selective stability increases by glycosylation of dihydrofolate reductase ppt
... two main types of protein glycosylation: N-glycosylation, in which the oligosaccharide is attached to an asparagine side chain, and O-glycosylation, in which it is attached to the side chain of ... modification in eukaryotic cells. It plays an important role in determining the properties of proteins, and stabilizes many proteins against thermal denaturation. Protein gly...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modified gold electrode pot
... effect. In the presence of catalase, a large increase in reduction current was observed upon addition of the substrate acetonitrile (Fig. 5), consis- tent with the substrate-induced passage of electrons ... A is the electrode area in cm 2 , C o is the enzyme concentration in the thin layer in molÆcm )3 and k f is the potential-dependent electron tra...
Ngày tải lên: 17/03/2014, 09:20