... humidified incubator containing 5% CO2at 37°C. The immortalized esophageal cell line NE-3was obtained from Dr. Jin (the University of HongKong, P. R. China)[26] and cultured in Keratinocyte-SFM (Invitrogen, ... Chinese Academy ofSciences, Shanghai, China) were grown in RPMI 1 640(Invitrogen, Carlsbad , CA) supplemented with 10% fetalbovine serum, 100 μg/L streptomycin, and 100 μg/Lpenicillin in ... CEA and CYFRA21-1 in these patients. In addition, sera from 41.0% of patients with ESCC were positive for CEA, SCC-Ag orCYFRA21-1, while sera from 64.2% of patients with ESCC were positive for...