Báo cáo sinh học: "Mutational analysis of the potential catalytic residues of the VV G1L metalloproteinase" pptx

... by immunoblot. Mutational analysis of the putative catalytic and zinc-binding residues of G1L utilizing a trans complementation assayFigure 4 Mutational analysis of the putative catalytic and zinc-binding residues of ... to wild type G1L and is the culmination of two independent experiments. With the exception of wild type G1L, the E120A mutant and, to a...

Ngày tải lên: 19/06/2014, 08:20

... complex, cleavage of the P 1 –P 1 ¢ peptide bond, linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand ... cognate proteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangem...

Ngày tải lên: 20/02/2014, 11:20

... indica- ted by the numbers on the right side. The migration of the protein markers is indicated by pencil marks on the membrane. The arrow indicates the position of the wild-type and N130D variants of human ... corrected by subtracting the spectrum of the buffer solution (5 mm Tris ⁄ HCl, pH 7.5) in the absence of protein. The stability to thermal inactivatio...

Ngày tải lên: 16/03/2014, 12:20

... a catalytic triade. No further efforts were made to track down the actual target of the inhibitors. DISCUSSION The purpose of t his work was to identify the key residues in the C-domain a nd their ... A milestone in the study of peptide s ynthetases was the determination of the A-domain structure of GrsA Phe -A [8]. Aspects of speci®city and binding were superi...

Ngày tải lên: 17/03/2014, 11:20

... Three of them (CRB-3–5) are in the vicinity of two putative coiled-coil domains, which suggests that they may participate together with the coiled-coil domains in folding of the N-terminal half of ... domains may position them in a way that they can contribute to the forma- tion of higher order structures of the Mrs2p part on the inner side of the membrane, which m...

Ngày tải lên: 23/03/2014, 10:21

... data not shown). On the basis of these results, we conclude that the absence of either of these two clusters of basic amino acid within the linker does not affect the ability of the protein to preferentially ... tyrosine on the scissile phosphate in the DNA, resulting in the cova- lent attachment of the enzyme to the 3¢ end of the broken strand [2]. Rotatio...

Ngày tải lên: 29/03/2014, 22:21

... site. Outcome 3 The final analysis determined whether the introduction of the CD4 nurse increased the monthly number of adult patients starting HAART. The outcome was the number of eligible patients ... including the study site, the study month (a meas- ure of the effect of time), the number of monthly MD/MO visits (a measure of MD/MO capacity), and the n...

Ngày tải lên: 18/06/2014, 17:20

... and increase in diversity of the T cell repertoire was detected in the pancreases, with most of the BV families present at pre-diabetic and d iabetic stages. A similar expansion profile of the BV TCR repertoire ... post-B cell reconstitution, the majority of the animals present pancreatic TCR expansions (at 13 weeks of life). Of note, these animals do not have clono- t...

Ngày tải lên: 18/06/2014, 19:20

... by immunoblot. Mutational analysis of the putative catalytic and zinc-binding residues of G1L utilizing a trans complementation assayFigure 4 Mutational analysis of the putative catalytic and zinc-binding residues of ... conditional mutant VV infection [26]. The role of both the full-length and cleaved products of VV G1L continue to be investigated as d...

Ngày tải lên: 20/06/2014, 04:20

... described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... the conversion of glutaminyl to pyroglutamyl residues. The hydrolysis reaction was monitored by calculating the sum of the relative intensities of the pea...

Ngày tải lên: 19/02/2014, 12:20