Báo cáo sinh học: "Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts" ppt

... is the first comparative study of all of the genomic compo- nents of the ORFs from UL146 through UL147A and the first report of multiple overlapping transcripts expressed from this region. The ... nucleotide sequences of the UL146- UL147A region, and in the case of CH1 and CH2 Northern analysis of temporal transcriptional pattern of UL14...

Ngày tải lên: 19/06/2014, 08:20

... genes, one from the HIV-1 Subtype B lab-adapted isolate NL4-3 and the other from a HIV-1 Subtype C primary isolate from India (called R5). The translated amino acid sequences (Fig. 1A) showed the predicted ... pGADT7. The NL and R5 vpu genes were cloned into the pGBKT7 and pGADT7 vectors as EcoRI- BamHI fragments from the pGEMT-Easy clones. The nucleotide sequenc...

Ngày tải lên: 18/06/2014, 18:20

... procedures, and regulatory requirements of the Institutional Animal Care and Use Committee, the Research Animal Resource Center of MSKCC, and the National Institutes of Health “Guide for the Care and ... monitoring of therapy is needed for human studies. The tracking of viral delivery could give clinicians the abil ity to correlate efficacy and therapy and...

Ngày tải lên: 18/06/2014, 19:20

... mea- sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and FCS ... Schematic diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudor coding sequences were joined to...

Ngày tải lên: 18/02/2014, 16:20

... fluorescence of tryptophan probes inserted in the L1 and L2 loop upon binding of nucleotides and DNA. The fluorescence of modified Rad51 was measured after the addition of the indicated element. The wavelength ... per monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less infl...

Ngày tải lên: 19/02/2014, 06:20

... acids at the N-terminus of the signaling sequence, but rather contained the L24ERGR motif at the C-terminus of the hydrophobic sequence, thereby resembling the R19ERQV, R19ERKV, R19VRQV and R19DRQ(S ... replicates. The N-terminal and C-terminal regions of several SDR enzymes were highly variable in length and sequence, and produced unreliable alignments. These r...

Ngày tải lên: 07/03/2014, 09:20

... define the RAIP motif [25]. The use of the rat protein also has the advantage that there is no cross-reactivity of the (P)Ser64 antibody with other sites, which is a compli- cating feature of the human ... the loss of raptor binding on removal of the first 17 residues may be accounted for simply by the loss of the RAIP motif. We therefore also tested the bind...

Ngày tải lên: 16/03/2014, 06:20

... in the maturation of the enzymes. Most o f them a ct pleiotropically in the synthesis of all four hydrogenases, in particular in the synthesis and insertion of the metal centre [6,7]. Two of the products ... besides being the educt for synthesis of the CN ligands, may also give rise to the formation of the carbonyl ligand [12]. The thiocarboxamide of the...

Ngày tải lên: 16/03/2014, 18:20