Báo cáo sinh học: " Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis" pdf

... contributions Design and conception of the study and co-drafted the manuscript (DPY); development of the methods and co- drafted the manuscript (EZ); assisted in the development of the automated plaque counting ... agreement and equivalence between traditional manual and automated plaque counting methods for detection of RSV neutraliz- ing antibody titers. The 180 te...

Ngày tải lên: 19/06/2014, 08:20

... Central Page 1 of 5 (page number not for citation purposes) Virology Journal Open Access Methodology Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque ... agreement and equivalence between traditional manual and automated plaque counting methods for detection of RSV neutraliz- ing antibody titers. T...

Ngày tải lên: 20/06/2014, 04:20

... responsible for cleavage of the A17 membrane protein, at an Ala-Gly-Ala site [9]. This consensus Ala-Gly-Xaa cleavage site of vaccinia is similar to that used for both the adenovirus and African swine ... Chelsea M Byrd - cbyrd@sgph.com; Dennis E Hruby* - dhruby@sgph.com * Corresponding author Abstract Through the use of transient expression assays and directed genetics, the vacc...

Ngày tải lên: 19/06/2014, 08:20

... the idea of the handbook and managed the project. HN conducted a detailed review of the literature and produced the first drafts of the handbook and this paper. SP provided case studies and technical ... quality of courses and ensure that courses remain relevant in the light of developing knowledge in the discipline. We stress the importance of having clear procedures for approv...

Ngày tải lên: 18/06/2014, 17:20

... (Dept. of Agriculture and Food, Republic of Ireland). Thank you to Mr. Noel Shanaghy and Mrs. Breda Doody of Waterford Regional Hospital, Mr. Eddie Beggan of Limerick Regional Hospital and Dr. ... slope of -3.5 and -3.7 respectively for GI and GII NoV (Fig. 1B and Fig. 2B). Melting curve analysis showed a positive peak at ~90°C and 88°C for GI and GII NoV. The broad reactivity...

Ngày tải lên: 18/06/2014, 18:20

... for analysis of therapeutics and candidate antibodies. It will be much more efficient for the purposes of quickly screening lead compounds and neutralizing antibodies than guinea pigs or nonhuman ... days postinfection showing presence of Marburg viral antigen (brown). Magnifications were 4× for panels A, C, and E, 20× for panels B, D, and F, and 40× for panels G-H. Virology...

Ngày tải lên: 18/06/2014, 18:20

... random assortment of thirty macaques housed at the WaNPRC and analyzed using the standard RV2 and OSM QPCR assays. While all of the samples were positive for the single copy OSM gene, only six of the thirty ... Contribution Design and conception of the study (AGB, TMR); develop- ment of the methods for amplification of the ORF59/60 regions (AGB, TMR); Development of the...

Ngày tải lên: 18/06/2014, 22:20

... usually uneven. Since failure of control measures is an important determinant of the distribution and spread of tuberculosis [29], it is important to focus trained human resources in areas of high tuberculosis ... detection. The urban slums of Moscow are populated by poor migrant workers from Kyrgyzstan and other Central Asian countries, many of whom contract tuberculosis due...

Ngày tải lên: 18/06/2014, 17:20

... insertion of the V3 region of an R5 isolate into an X4 molecular clone altered the tropism of an X4 isolate to an R5 phenotype [10,19,23,28-30]. Our in-silico analysis of the V3 sequences of the ... India. We thank AIDS Reference and Reagent Program for providing HIV-1 isolates and cell lines and Roshni Mehta and Brian Wellensiek of Ahmad Lab for critically reviewing...

Ngày tải lên: 18/06/2014, 18:20

... investigation conformed to the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guide for the Care and Use of Laboratory Animals published by the U.S. ... on a scale of 0-18 (normal score 0, maximal deficit score 18) Statistical analysis Data were presented as mean values and standard devia- tion. A method of ANOVA (analysis...

Ngày tải lên: 18/06/2014, 19:20