... JournalOpen AccessMethodology An inexpensive and rapid diagnostic method of Koi Herpesvirus (KHV) infection by loop-mediated isothermal amplificationHatem Soliman and Mansour El-Matbouli*Address: ... disease struck koi population in the USA and Israel in1998 and spread rapidly [5]; it has been reported in Ger-many [6], Korea [7,8], Indonesia [9], Japan [10], SouthAfrica, and Thailand (unpublished ... data).Clinical signs of KHV are often non-specific and mortalitymay occur rapidly. Discoloration and severe necrosis of the gills is the most consistent sign of infection, with dis-orientation and erratically...