Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf

... decreases in detectible RNA. Effect of incubation temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the ... while maximizing the ability to degrade carry-over amplicon DNA contamination in a sample. Results Effect of UNG concentration on DNA...

Ngày tải lên: 19/06/2014, 08:20

... temperature and time on DNA degradation and RT-PCR amplification of RNA The effect of temperature on DNA degradation and RNA amplification in the presence of UNG was assessed at three incubation temperatures ... while maximizing the ability to degrade carry-over amplicon DNA contamination in a sample. Results Effect of UNG concentration on...

Ngày tải lên: 20/06/2014, 04:20

... IL-13Ra2-negative tumors. Conclusions: We have identified a novel function of histone modification in the regulation of IL-13Ra 2in pancreatic cancer cell lines in vitro and in vivo. HDAC inhibition provides ... opportunity in designing combinatorial therapeutic approaches no t only in combination with IL-13-PE but with other immunotoxins for therapy of pancreatic cancer...

Ngày tải lên: 18/06/2014, 19:20

... grading. The study was conducted in accordance with the Helsinki Declaration and approved by the Ethic s Committee of our institution. Patients were informed of the investiga- tional nature of the ... progression by regulating the immune microenvironment of regional- draining lymph nodes, this study also examined the mast cell count in the regional-draining lymph...

Ngày tải lên: 18/06/2014, 19:20

... participated in the analysis of the results and drafted the manuscript. AV participated in the analysis of the results and helped to draft the manu- script. AP designed the study, participated in the ... cross-neutralization tests [4], and the coexistence of SAAV and DOBV in the same geographic region [5,6] indicates reproductive isolation. They also...

Ngày tải lên: 19/06/2014, 08:20

... task (standing quietly). By focusing attention on the task of standing still, subjects may have artificially constrained the interactions among underlying postural control system components, thereby ... citation purposes) the subject's predetermined (seated) digit span. Upon reciting the final digit in the string, the examiner initiated COP recording, and the subjec...

Ngày tải lên: 19/06/2014, 10:20

... prevention and control for Zimbabwe. These included expansion of Prevention of Mother to Child Transmission of HIV (PMTCT), expansion of HIV testing capacity, improved understanding of the epidemic through ... strength and resources of many partners to respond more effectively to the HIV/ AIDS epidemic by reinforcing a crucial training institution and thereby expanding p...

Ngày tải lên: 18/06/2014, 17:20

... modulations of enzyme activities. In particular, in the combinations involving the decrease of glyoxalase II activity the effect is equival- ent to the modulation of the other parameters alone, as ... trypanothione concentration. In all other combinations there is only a slight effect on methylglyoxal concentration suggesting that a signi- ficant increase of this...

Ngày tải lên: 23/03/2014, 13:20

... the elimination of avoidable blindness launched jointly by the World Health Organization and the International Agency for the Prevention of Blindness with an international membership of nongovernmental ... Interna- tional Agency for the Prevention of Blindness with an international membership of nongovernmental organiza- tions, professional associations, eye care institu...

Ngày tải lên: 18/06/2014, 17:20

... collection and interpretation. PG provided intellec- tual and theoretical input for the paper and interpretation of the findings. All authors were involved in revising the manuscript and have read and ... modelling The final stage of analysis focuses on the modelling of intentions and working as a nurse. The relationships between intentions expressed at earli...

Ngày tải lên: 18/06/2014, 17:20