Báo cáo sinh học: " Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription" potx

... dissociation of the Tat- N -TAR com- plex, and lanes 8–13 show the dissociation of the Tat- P -TAR complex. Lane 1 is TAR RNA only. Tat- N Tat- P Tat- TAR 12345678 910111213 Tat- N Tat- P Tat- N Tat- P + cold TAR Tat- TAR Free ... ng of either Tat- N or Tat- P, and approximately 70 000 cpm of 32 P -TAR RNA (lanes 1 and 2), or approximately 70 000 cpm...

Ngày tải lên: 18/06/2014, 22:20

... citation purposes) Phosphorylation of HIV- 1 Tat8 6 by PKRFigure 1 Phosphorylation of HIV- 1 Tat8 6 by PKR. (a) PKR was immunoprecipitated from HeLa cell extracts and activated with synthetic dsRNA ... is TAR RNA only. Tat- N Tat- P Tat- TAR 12345678 910111213 Tat- N Tat- P Tat- N Tat- P + cold TAR Tat- TAR Free TAR a b Virology Journal 2005, 2:17 h...

Ngày tải lên: 20/06/2014, 04:20

... Comparative rates of entry of chimeras correspond with the peak of viral replication, where 171 and 173 with highest level of entry in MAGI cells showed very early and high peaks and 284 and 331 chimeras with ... patient sample and these are numbered with numerals of patient identification number followed by clone number. All the clones were again checked by di...

Ngày tải lên: 18/06/2014, 18:20

... reactive with HIV- 1 and/ or HIV- 2 antigens using the HIV- 1AB HIV- 1IHIV-2 (rDNA) EIA kit (HIV- BIA, Abbott Laboratories) and HIV- 1 and HIV- 2 Wb assays (Cambridge Biotech, Rockville, Maryland) accord- ing ... High proportion of HIV- 2 and HIV- 1/ 2 double-reactive sera in two Indian states, Maharashtra and Goa: first appearance of an HIV- 2 epidemic along with a...

Ngày tải lên: 19/06/2014, 08:20

... as conformational change of each of His-CI and CTD at 42°C compared to those at 30°C. CD-spectra of His-CI and CTDFigure 2 CD-spectra of His-CI and CTD. Far UV CD-spectra of His-CI and CTD (64–183 amino ... repression of early promoters and stably maintains lysogeny [7]. Interestingly, the mechanism of actions of repressors of coliphages P1 and P7 [8,9], mycobac...

Ngày tải lên: 18/06/2014, 18:20

... Acknowledgements

Ngày tải lên: 18/06/2014, 18:20

... mechanisms of interaction. One of those mechanisms involves a bridg- ing function in which CBP links c-Myb with components of the basal transcription machinery, thereby establishing and/ or stabilizing ... of JAK/STAT and Ras/AP-1 signaling by CBP and p300. Proc Natl Acad Sci U S A 1997, 94(4):1074-1079. 29. Takahashi T, Suwabe N, Dai P, Yamamoto M, Ishii S, Nakano T: Inhib-...

Ngày tải lên: 18/06/2014, 22:20

... 1 and 2 were inoculated with 10 7 EID 50 /0.2 ml of inactivated and attenuated SPPV vaccine, respectively by i.p. route of inoculation. Mice in group 3 were kept as a placebo and inoculated with ... amounts of SPPV induced enhancement of IL-12 due to ability of IL- 12 to induce early phase of NK and T cell activation [34]. Using CRBC as model of TD Ag, it has been...

Ngày tải lên: 18/06/2014, 22:20

... 5 and 20 l M of benomyl treatment (lanes 2 and 3); and 40 h after 5 and 20 l M of benomyl treatment (lanes 4 and 5). Fig. 9. Benomyl induced hyperphosphorylation of bcl2 and dissoci- ation of ... Cleavage of PARP by benomyl treatment. HeLa cells were treated with the indi- cated concentrations of drugs for 40 h and equal amounts of cell lysates were resolved b...

Ngày tải lên: 30/03/2014, 10:20

... V600E and V600K mutations. Our preclinical data demonstrating s imilar kinase activity of the V600K and V600E mutations, together with clear evidence of clinical activity of PLX4032 in a patient with a ... Rubinstein et al.: Incidence of the V600K mutation among melanoma patients with BRAF mutations, and potential therapeutic response to the specific BRAF inhibitor PLX4032...

Ngày tải lên: 18/06/2014, 16:20