Báo cáo sinh học: " Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription" potx
Báo cáo sinh học: " Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription" potx
... dissociation of the Tat- N -TAR com-plex, and lanes 8–13 show the dissociation of the Tat- P -TAR complex. Lane 1 is TAR RNA only. Tat- N Tat- P Tat- TAR 12345678 910111213 Tat- N Tat- P Tat- N Tat- P+ cold TAR Tat- TAR Free ... ng of either Tat- N or Tat- P, and approximately 70 000 cpm of 32P -TAR RNA (lanes 1 and 2), or approximately 70 000 cpm of 32P -TAR RNA and 7.5 × the volume of unlabelled TAR RNA (lanes 3 and ... BioMed CentralPage 1 of 13(page number not for citation purposes)Virology JournalOpen AccessResearch Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcriptionLiliana...
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báo cáo hóa học:" Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcription" ppt
... citation purposes) Phosphorylation of HIV- 1 Tat8 6 by PKRFigure 1 Phosphorylation of HIV- 1 Tat8 6 by PKR. (a) PKR was immunoprecipitated from HeLa cell extracts and activated with synthetic dsRNA ... is TAR RNA only. Tat- N Tat- P Tat- TAR 12345678 910111213 Tat- N Tat- P Tat- N Tat- P+ cold TAR Tat- TAR Free TAR abVirology Journal 2005, 2:17 http://www.virologyj.com/content/2/1/17Page 7 of 13(page ... number not for citation purposes) PKR phosphorylation of HIV- 1 Tat wild-type and mutantsFigure 4 PKR phosphorylation of HIV- 1 Tat wild-type and mutants. HIV- 1 Tat wild-type and mutant proteins...
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Báo cáo sinh học: " Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages" docx
... Comparativerates of entry of chimeras correspond with the peak of viralreplication, where 171 and 173 with highest level of entryin MAGI cells showed very early and high peaks and 284 and 331 chimeras with ... patientsample and these are numbered with numerals of patientidentification number followed by clone number. All theclones were again checked by digestion with EcoRI/SalI and BamHI and confirmed by sequencing.Cell ... syncy-tium-inducing (SI) virus (HIV- 1NL4-3) and a non-syncytium-inducing (NSI) viruses (HIV- 1BaL and HIV- 1Ada-M) by infect-ing the MT-2 cell line with equal amounts of these viruses.Syncytium...
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Báo cáo sinh học: " Evidence of HIV exposure and transient seroreactivity in archived HIV-negative severe hemophiliac sera" docx
... reactive with HIV- 1 and/ or HIV- 2antigens using the HIV- 1AB HIV- 1IHIV-2 (rDNA) EIA kit (HIV- BIA, Abbott Laboratories) and HIV- 1 and HIV- 2 Wbassays (Cambridge Biotech, Rockville, Maryland) accord-ing ... High proportion of HIV- 2 and HIV- 1/2 double-reactive sera in two Indian states, Maharashtra and Goa: first appearance of an HIV- 2 epidemic along with an HIV- 1 epidemic outside of Africa. Zentralbl ... Laboratories). HIV- 1 and HIV- 2 Wb HIV- 1 and HIV- 2 Wb analysis was performed using nitro-cellulose strips containing electrophoretically separated and transferred proteins from inactivated HIV- 1 or HIV- 2lysates....
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Báo cáo sinh học: " Repressor of temperate mycobacteriophage L1 harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity" ppt
... asconformational change of each of His-CI and CTD at42°C compared to those at 30°C.CD-spectra of His-CI and CTDFigure 2CD-spectra of His-CI and CTD. Far UV CD-spectra of His-CI and CTD (64–183 amino ... repression of early promoters and stably maintains lysogeny [7]. Interestingly, themechanism of actions of repressors of coliphages P1 and P7 [8,9], mycobacteriophages L5 and Bxb1 [10], and B.subtilis ... aminoacid residues of CI) amino acid residues of His-CI. As thesize of fragment c appeared unchanged over the wholedigestion period and fragment d and e disappeared with increasing time of digestion,...
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Báo cáo sinh học: " Selection of Recombinant MVA by Rescue of the Essential D4R Gene" doc
... Acknowledgements ...
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Báo cáo sinh học: " Regulation of FeLV-945 by c-Myb binding and CBP recruitment to the LTR" doc
... mechanisms of interaction. One of those mechanisms involves a bridg-ing function in which CBP links c-Myb with components of the basal transcription machinery, thereby establishing and/ or stabilizing ... of JAK/STAT and Ras/AP-1 signaling by CBP and p300. Proc Natl Acad Sci U S A1997, 94(4):1074-1079.29. Takahashi T, Suwabe N, Dai P, Yamamoto M, Ishii S, Nakano T: Inhib-itory interaction of ... for hematopoiesis and known to be present in limiting amounts. Interaction of c-Myb and CBP with the 21-bp triplication was shown topositively regulate virus production, and thus may beresponsible...
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Báo cáo sinh học: " Modulation of macrophage functions by sheeppox virus provides clues to understand interaction of the virus with host immune system" docx
... 1 and 2 were inoculated with 107EID50/0.2 ml of inactivated and attenuated SPPV vaccine,respectively by i.p. route of inoculation. Mice in group 3were kept as a placebo and inoculated with ... amounts of SPPV induced enhancement of IL-12 due to ability of IL-12 to induce early phase of NK and T cell activation [34].Using CRBC as model of TD Ag, it has been shown thatSPPV was capable of ... =A2-A0/A1-A0. A2 is the absorption of cultures with PHA-P; A1 is the absorption of cultures without mitogen; A0 isabsorption of the blank (culture medium only).Analyses of IL-12 and SOD from cultured splenocytesThe...
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Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
... 5 and 20 lM of benomyl treatment (lanes 2 and 3); and 40 h after 5 and 20 lM of benomyl treatment (lanes 4 and 5).Fig. 9. Benomyl induced hyperphosphorylation of bcl2 and dissoci-ation of ... Cleavage of PARP by benomyl treatment. HeLa cells were treated with the indi-cated concentrations of drugs for 40 h and equal amounts of celllysates were resolved by SDS ⁄ PAGE followed by immunoblotting with ... the hyper phosphorylated form of bcl2 and cleavage of PARP [36,48]. Both the floating and attached cells were harvested with the help of a cellscraper and collected by centrifugation. The cells...
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Báo cáo hóa học: " Incidence of the V600K mutation among melanoma patients with BRAF mutations, and potential therapeutic response to the specific BRAF inhibitor PLX4032" potx
... V600E and V600K mutations. Ourpreclinical data demonstrating s imilar kinase activity of the V600K and V600E mutations, together with clearevidence of clinical activity of PLX4032 in a patient with a ... Rubinstein et al.: Incidence of the V600K mutationamong melanoma patients with BRAF mutations, and potentialtherapeutic response to the specific BRAF inhibitor PLX4032. Journal of Translational Medicine ... publication of this case report and accompanying i mages. A copy of the written consent is available for review by the Editor-in-Chief of this journal. Upon retesting for BRAFTable 1 Incidence of V600...
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