Báo cáo sinh học: " Regulation of FeLV-945 by c-Myb binding and CBP recruitment to the LTR" doc

... cleavage seen in our experiments. The observed translocation of ADAM9 and ADAM10 to the cyto- plasmic membrane further supports the idea of APP cleavage at the membrane by these secretases. Our experiments ... (Figs S5 and S6A) and protein levels of BACE1 (Figs S5 and S6C) were accompanied by its impaired trafficking and late translocation to the cellular memb...

Ngày tải lên: 16/03/2014, 01:20

... attempted to identify the apoptotic fac- tor(s) that were regulated by DNp63a in response to TNF-a. TNF-a is known to trigger apoptosis by diverse mechanisms, including caspase activation and the mitochondrial ... TAp63 ⁄ TAp73, and the silencing of DNp63a allows for the induction of the proapoptotic genes Bax, Noxa, and Puma [33]. Therefore, we employed real tim...

Ngày tải lên: 16/03/2014, 06:20

... esta- blishment of spermatogenesis and development of the adult pattern of testicular function. Our data indicated that the regulation of HSPG mRNA expression is HSPG-specific and depends on the Sertoli ... stimulated them by +35 and +55%, respectively (Tables 2 and 3). DISCUSSION This report shows, for the first time, the FSH regulation of HSPG mRNA expression...

Ngày tải lên: 17/03/2014, 23:20

... for the hyperacute vascular rejection occurring in pig -to- primates organ trans- plantation. The synthesis of the aGal epitope is catalyzed by the enzyme a1,3-galactosyltransferase (a1,3GalT). To ... resting and TNFa-stimulated pig endothelial cells. The strong homology of exon 0 to a portion of the porcine invariant chain gene did not allow to d esign p rimers sp...

Ngày tải lên: 08/03/2014, 16:20

... compared the values of DG° t to the apparent binding energies of Na + and K + in pyruvate kinase. For the latter calculations, we assumed that the K 0.5 app is equal to the K d , and from the equilibrium constants ... energetics of binding, we have explored if the magnitude of the transfer energies of Na + and K + from water to dimethylsulfoxide is rel...

Ngày tải lên: 08/03/2014, 02:20

... occurs by a two-step mechanism in which the N-terminus of the inhibitor first binds to the enzyme, displacing the occluding loop, followed by the binding of another two loops of the inhibitor [24]. Besides ... determine the interference with the binding of substrates and other inhibitors and to iden- tify the mechanism by which it regulates the act...

Ngày tải lên: 18/02/2014, 11:20

... hyperbolic binding of ligands to the enzyme N ¼ N max ½L=ðK d þ½LÞ ð3Þ where N is the degree of binding with the dissociation con- stant K d of ligand L to the enzyme with a maximal number of binding ... regulatory point of view. dCMP deaminase is activa- ted by dCTP and inhibited by dTTP and both nucleo- tides act on the enzyme by binding to an a...

Ngày tải lên: 18/02/2014, 16:20

... solution. On the other hand, the acceleration of the sliding velocity (and the lower number of stops) of thin filaments could be related to a diminution o f the load in the actin–myosin interface. The ... results in the inhibition of HMM cross- linking to the N-terminal part of a ctin. This change of the actin–HMM ionic interface would be in contrast to...

Ngày tải lên: 19/02/2014, 16:20

... P2 and P3 indicate the probes used for EMSAs. (C) Binding of transcription factors to the Dnmt1 promoter. Binding of transcription factors to the region between nucleotides )173 and )117 of the ... motif) and the binding of each is independent of the other (Fig. 5). A competition experiment in vitro with the GA motif demonstrated that each factor is abl...

Ngày tải lên: 22/02/2014, 07:20

... [16]. The lat- ter is in turn used by ODC to produce putrescine, the precursor of spermidine and spermine. To further ana- lyze the regulation of the polyamine-synthetic pathway in virus infection, ... polyamine in the regulation of apop- tosis [21,22]. Thus, the possibility that the antiviral effect of spermine is mediated by induction of apop- tosis was...

Ngày tải lên: 07/03/2014, 21:20