... Another function of AclB was found to be stabilization of the enzyme, as AclB prevented the degradation of AclA that was otherwise observed in the absence of AclB. After the phosphorylation, ... mechanism of mammalian ACL [23], the final step of the reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated carbonyl carbon of citryl phosp...
Ngày tải lên: 08/03/2014, 22:20
... [3]; and from the deconvolution of CD at 37 °C in the absence of NaCl (195– 260 nm) was 51%. For the spectrum of TOP in the same conditions the deconvolution indicated 45% of a helix content. This ... Val position in order to verify the influence of the nature of the amino acid at this position on determination of the cleavage sites and on the amount of...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... render the potential sites of PKC and PKA phosphorylation on the claudin-1 pro- tein non phosphorylatable or to mimic constitutive activation of PKC or PKA. B) Rendering the serine at position ... mutating the PKC phosphorylation sites to mimic a non-phosphorylated state did not cause a shift of claudin-1 to the nucleus of the cells, but mutating the PKA sites did...
Ngày tải lên: 03/11/2012, 11:17
Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... modu- lation of Ca 2+ oscillations by increasing agonist con- centration [44]. The dependences of the period of oscillations on the RPTP and Csk concentrations almost mirror each other, although there ... from the Off state to On state (in the bistability domain). Regardless of the slope, the active Src fractions increase during the Off to On transition, whereas...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... is the case for almost all DNA glycosylases [47], the preferences for each sub- strate may depend on the reaction conditions to differ- ent degrees. The influence of the reaction conditions on ... to induce conformational transitions in nucleic acids, pos- sibly by selective stabilization ⁄ destabilization of one of the conformations; for example, submillimolar concen- tr...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf
... ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc- ture as the desired output of the morphological analysis, ... independently from other boundaries (zero- order) and the graphemic representation of the word, however, is conditioned on the length of the word...
Ngày tải lên: 20/02/2014, 04:20
Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... presented and on this basis the contributions of the reorganization of the polypeptide backbone, of individual amino acids and of the hemes c, a and a 3 upon electron transfer to/from the redox active ... to the different environ- ment of the heme centers. The difference signals observed at 442 nm and 603 nm can be assigned to the contributions of the hemes a...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... However, the mechanisms involved in the down-regulation of fat oxidation by NPs require further investigation. Fat accumulation and the down-regulation of the b-oxidation of FFAs were induced in PC12 ... that the degree of inter- nalization of QDs and various other NPs is dependent on their size, surface charge, concentration in the med- ium and duration of exposure...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... depend strongly on the temperature [53,54]. Indeed, the change in the array of the hydrogen bonds at thymine of SRE GGfos is probably a sign of perturbation in the hydration scheme along the minor ... information on the local repartition of the angles involved in the SRE fos helix bending. Relative effect of bending strain There are several indications of a r...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... in the position of the pyrimidine ring, whereas the phos- phate and phosphonate moieties occupy the same posi- tion in spite of the different conformation of the alkyl chain. The positions of the ... inhibitors. The centre of the pyrimidine moi- ety is located in a position which corresponds to the position of the common bond between the two rings in the...
Ngày tải lên: 07/03/2014, 11:20