... in L. lactis code for a functional chitinolytic machinery capable of converting chitin to GlcNAc and (GlcNAc) 2 . The primary product of this machinery is (GlcNAc) 2 , which can be converted to mono-sugars ... (forward primer, 5¢-GGATGAGCTCTATACTCACATCTTGAGC- 3¢; reverse primer, 5¢-TTGTGGGCCCAACCAATCTATG AAGAATT-3¢). The PCR product was cloned using the zero blunt TOPO-cloning kit (Invitrog...
Ngày tải lên: 18/02/2014, 08:20
... 5¢-ATGCATGGTACCTCAG GCCAGATCAAGGCCGCG-3¢. All constructs were main- tained in Escherichia coli DH5 and were confirmed by sequence analysis using the DYEnamic ET terminator cycle sequencing kit (Amersham Biosciences). ... eukaryotic cell. It is apparent that more secrets concerning this intriguingly complex interaction need to be uncovered. Many of these may be revealed only through compre-...
Ngày tải lên: 19/02/2014, 08:20
Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... 5¢-CATGGCTTAATCTTTGAGAC-3¢ [8] P3 5¢-GCTCTCATGCTCTTGCCAAAAC-3¢ [9] P4 5¢-CGTATCGCATTTCGCTGCGTTC-3¢ [9] P5 5¢-CTCACTACCAAACAGAATGTTTGAGAAGG-3¢ [13] P6 5¢-GTTCGCCTAGACGCTCTCTTC-3¢ [9] P7 5¢-GCCGCTTCACTCGCCGTTACTAAGGC-3¢ ... After precipitation, the recovered RNA was denatured and separated by electro- phoresis on 6% polyacrylamide or 1.5% agarose gels and transferred to nylon membranes. For...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... Chemistry, Faculty of Agriculture and 2 Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Japan A bacterial strain that grew on 4-amino-3-hydroxybenzoic acid ... 4-amino-3-hydroxybenzoic acid. The purification and characterization of a dioxygenase from this strain, Correspondence to K. Aoki, Department of Biofunctional Chemistr...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
... transcription cycle, R NAP makes speci c and nonspeci c contacts with double and single stranded (ss) DNA, the RNA/DNA hybrid and nascent RNA. Recent advances in structural studies of bacterial ... for the synthesis of all types of cellular RNAs. The process of transcription is accompanied by complex structural rearrangements of RNAP. Despite the recent progress in structural studie...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: "Automatic training of lemmatization rules that handle morphological changes in pre-, in- and suffixes alike" docx
... Incorrect Correct (supporters) Correct N wr N rr Incorrect N ww N rw Not matched N wn N rn Sum N w N r Table 2. The six parameters for rule selection among candidate rules. ... three correctly) and getalmd (incorrectly) while the second rule handles words like directeur (correctly) and zei (incor- rectly). 3.2 Inflected vs. agglutinated languages A lemmatizer...
Ngày tải lên: 23/03/2014, 16:21
Báo cáo khoa học: "A MODEL OF PLAN INFERENCE THAT DISTINGUISHES BETWEEN THE BELIEFS OF ACTORS AND OBSERVERS" pot
... INFERENCE THAT DISTINGUISHES BETWEEN THE BELIEFS OF ACTORS AND OBSERVERS Martha E. Pollack Artificial Intelligence Center and Center for the Study of Language and Information SRI International ... general theory of action and time. Artificial Intelligence, 23(2):123-154, 1984. [4] Michael Bratman. Intention, Plans and Practical Reason. Harvard University Press, Cambridg...
Ngày tải lên: 31/03/2014, 17:20
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... mice were housed at 23-25 C in a 12-hour light/dark cycle with access to standard powdered mice chow and normal water. The scientific project, including animal care was supervised and approved ... three groups: normal control group, diabetic control group, and diabetic treated group. Each group included 8 mice. Diabetes was induced in mice by a single intraperitoneal injection o...
Ngày tải lên: 25/10/2012, 11:10
Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"
... Alessandro R, Belluco C, Kohn EC. Proteomic approaches in colon cancer: promising tools for new cancer markers and drug target discovery. Clin Colorectal Cancer. 2005; 4(6):396-402. 35. Cho WC. ... Abstract The aim of present study is to study the serum protein fingerprint of patients with colorectal cancer (CRC) and to screen protein molecules that are closely related to colore...
Ngày tải lên: 25/10/2012, 11:18
Báo cáo y học: " Rationale for one stage exchange of infected hip replacement using uncemented implants and antibiotic impregnated bone graft"
... Patrick S, Curran MD, Ramage G, Hanna D, Nixon JR, Gorman SP, Davis RI, Anderson N. Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification ... Davis RI, Anderson N. Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Mi...
Ngày tải lên: 26/10/2012, 09:53