... The sequence corresponding to the N-terminal sequence of the protein is underlined. The arrowheads indicate the positions of the intron sequences. (B) Comparison of the amino-acid sequences of ... Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the active site of the C. sepium b-amylase most probably consists of a cleft located betwe...
Ngày tải lên: 22/02/2014, 07:20
... published elsewhere. We have therefore measured the thermodynamic parameters of RNase-A in the presence of these amino acids and amino acid derivatives, and values of DG D °, measured in triplicate, ... those in the absence of sugars, i.e. K m is decreased. On the other hand, this change in conformation results in a decrease in k cat , the turnover number of the enzy...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... mutations in either pilW or pilQ significantly affect the location of the other protein in the outer membrane. Furthermore, no PilA4 was present in the outer membranes of these mutants. From these findings, ... that the nonconserved PilW pro- tein is implicated in the assembly and stability of PilQ multimers at the inner membrane and transport of these subassemblies to...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... chain conformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b 5 is 0.21 A ˚ , within the error limit. The side chain of Tyr35 of the ... [35,36,40,41]. The apo- cyt b 5 and apo-Mb have no absorption in the Soret band region because of the lack of the heme prosthetic group. For...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt
... inwards, the arms close and the tips of the arms wrap around the DNA. The amplitudes and time scales of the intramolecular motions experienced by the protein backbone are com- monly determined from the 15 N-NMR ... rigid parts of MC1, such as the a-helix and the b-strands, excluding bulges. The correlation functions for two residues, Val18 in a bulge and As...
Ngày tải lên: 22/03/2014, 17:20
Báo cáo khoa học: De novo synthesis, uptake and proteolytic processing of lipocalin-type prostaglandin D synthase, b-trace, in the kidneys pptx
... treatment, the two forms of monkey L-PGDS migrated to positions of M r = 18 000 and M r = 19 000, the latter of which corresponds to the non-glycosylated form of L-PGDS [7], and both of which were ... of b-trace with K d values of 91 and 2900 nm, respectively, and to the recombinant D 1)22 Cys 65,167 Ala L-PGDS with values of 167 and 57 nm, respectively. The...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Submembraneous microtubule cytoskeleton: biochemical and functional interplay of TRP channels with the cytoskeleton pot
... remarks The last few years have seen rapid progress in the study of TRP channels as well as other ion channels in the context of both actin and the microtubule cytoskel- eton. The presence of the ... morphology and motility, as well as the transport of molecules and organelles toward and from the cell membrane. Although all these phenomena affect the plasma m...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... for the best overlap of the backbone of residues 280–295 and superimposed on the lowest energy structure. This image was constructed with the VMD software [36]. Fig. 6. NMR structure of the amphipathic ... protons in the b turn (Fig. 3B). However, further detailed structural descriptions of the aggregate are needed to better understand the stabilizing function of...
Ngày tải lên: 31/03/2014, 21:21
Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf
... insertions 751 Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754, Suntec, Singapore, 2-7 August 2009. c 2009 ACL and AFNLP What lies beneath: Semantic and syntactic ... that of Chomskian theory or Harris’s concep- tion of transformation (Harris, 1957). In this view, the original verbatim string is the surface form of...
Ngày tải lên: 20/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Prosodic Aids to Syntactic and Semantic Analysis of Spoken English" ppt
... purpose of utteranc- es and their domain context, it is then possible to correct some of the insertion and mis-recognised word errors from the SR and determine the com- municative intent of the ... modified the grammar they use to change all the rules of the form A -> B C to the form A -> B Link C, and add con- straints to the rules application i...
Ngày tải lên: 20/02/2014, 21:20