báo cáo hóa học: " Health-related quality of life in young adults with symptoms of constipation continuing from childhood into adulthood" potx
Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... [8,9]. BTB ⁄ POZ proteins areoften transcriptional regulators containing a C2H2domain for DNA binding, but can also be found in combination with various other protein–protein inter-action motifs, ... aworking model in which they bind to ERF ⁄ AP2s,potentially interfering with their DNA-binding ability,but which ultimately results in the degradation of ERF ⁄ AP2 proteins ( Fig. 6). Independent ... assembly with CUL3 and other BTB proteins [2,16], it remains unclearwhat kind of interactions are mediated by the MATHdomain in plants. In this article, we show that the MATH domain of BPM proteins...
- 12
- 657
- 0
Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... resulting in elucidation of a new activity of sili-catein as a silica esterase, we used recombinant silica-tein-a from the demosponge S. domuncula [10].ResultsPresence of silicatein in the ... thespicules in concentric rings (Fig. 2B). A closer view of the axial canal in the center of the spicule reveals local-ization of silicatein in the axial filament as well aswithin the silica ... enzymatically via silicatein [7,8].Based on its protein sequence, silicatein is related tothe proteinases of the cathepsin class [9].Silicatein has been isolated from a number of sili-ceous sponges,...
- 9
- 576
- 0
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... pretreated with the PKA inhibitors, KT5720 and H89 [28]. Followingsaponin permeabilization, this resulted in increasedInsP3-induced Ca2+release with either inhibitor, with an EC50 of 1 lm ... (B) Initial part of the same thrombin generation curves. (C) Bars indicatethe effects of preincubation on initial rates (5 min) of thrombin generation (n = 3–5). (D) Human platelets in fibrin-depleted ... examinedusing two structurally distinct inhibitors, wortmanninand LY294002 [21]. In the presence of ADP ⁄ P2Y12activity, wortmannin or LY294002 suppressed thethrombin-induced Ca2+integral...
- 15
- 565
- 0
Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx
... substrate carbinolamine intermediate;III, iminoquinone intermediate; IV, product Schiff base intermediate;V, aminoquinol intermediate. In the oxidative half-reaction the ami-noquinol intermediate ... kinetic properties of the recombinant AADHwere indistinguishable from those of the native enzyme isolated from A. faecalis. TTQ biogenesis in recombinant AADH is functional despitethe lack of ... pKavalue of 6.0, with maximum activity realized in the alkaline region. Two kinetically in uentialionizations were identified in plots of klim⁄ Kd of pKavalues 7.1 and 9.3,again with the...
- 16
- 605
- 0
Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... formation.Incubation of Cys124 peptide with BB1 indeed resulted in a35% inhibition of amyloid fibril formation.Our results are in keeping with the clinical observations of Arg124 mutation-linked amyloidosis ... for integrins. The particularintegrins with binding capacities for the corneal TGFBIprotein remain to be fully identified. Kim et al. [3] haverecently shown that alpha3-beta1 integrins bind ... scale using acontinuous flow apparatus (PE Biosystem, Pioneer,Framingham, USA) starting from Fmoc-PAL-PEG-PSresins. The coupling reaction was performed with 0.5M of HATU in presence of 1M of DIEA....
- 8
- 469
- 0
Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... residue (Trp677 in rat,Trp676 in human CPR) stacks against the isoalloxa-zine ring of the FAD cofactor thereby preventinghydride transfer from NADPH to the flavin-N5 andthus necessitating a potentially ... transfer during the stoichiometricreduction of CPR with NADPH.Summary of resultsScheme 2 outlines possible reasons for the diminishingintermediate formation of blue di-sq at increasing pH,when ... pH gating mecha-nisms. We were principally interested in the inter-flavinET reactions, so the pH dependence of the kineticbehaviour at 600 nm was analysed, reporting on theformation of the...
- 18
- 414
- 0
Báo cáo khoa học: Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea potx
... thesulfuration of the C-terminal glycine in ThiS andMoaD, involved in thiamine biosynthesis and molyb-dopterin biosynthesis, respectively, and for the sulfura-tion of uridine in tRNA to thiouridine can ... containing N-methylhistidine, 5-methyl-arginine or 2-methylglutamineThe methylated histidine (H257) was found in the a-sub-unit of MCRs from all methanogenic archaea and of thetwo MCRs from ... phenylalanine instead of atyrosine directly before the thioglycine (Methanoculleusthermophilus and Methanosarcina barkeri) or directlyafter the thioglycine (Methanosarcina barkeri), and analanine instead...
- 9
- 548
- 0
Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
... separately fittingdomains I and II to the structure of DcP. The identification of the appropriate clipping points was aided by using theAlternate Domain Fit tool from the suite of tools withinthe swiss-pdbviewer ... impli-cated in substrate binding, are shown in Fig. 3 to bewithin hydrogen bonding distance from the substrate.As energy minimization only allows for limited con-formational sampling, we also ... two lobes. Modeling TOPonto DcP moved several domain II Tyr residues of TOP, known to be involved in catalysis or substratebinding [17,19], into positions analogous to those of closed DcP, and...
- 11
- 395
- 0
Báo cáo khoa học: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of b-oxidation doc
... with 10% fetalbovine serum (Fig. 5D) followed by staining with OilRed O showed a significant increase in lipid droplets in comparison with Ad.CMV.GFP-infected cells(Fig. 5C). In agreement with ... and then with a solution of 50 mmNH4Cl in NaCl ⁄ Pi. Slides were washed with NaCl ⁄ Piandincubated in NaCl ⁄ Picontaining 5% BSA for 30 min. Forimmunostaining, the slides were incubated ... Cells infected with 500 m.o.i. of Ad.CMV.GFP then incuba-ted in RPMI-1640 supplemented with 100lgÆmL)1VLDL. (F) Cells infected with 500m.o.i. of Ad.CMV.adipophilin then incubated in RPMI-1640...
- 13
- 349
- 0
Báo cáo y học: "Bench-to-bedside review: The MET syndrome – the challenges of researching and adopting medical emergency teams"
... promotes tracking effectsover time, integrating detailed process knowledge into thework of interpretation, using small samples and short experi-mental cycles of change, and using multifactorial ... examples of before-and-after studies dealing with single-centre data[52-56]. Inherent within this type of evidence is the lack of rigour and generalisability. Furthermore, the magnitude of theeffect ... times more frequent than late signs, with nurses accounting for 86.1% of these [45]. Interestingly, in that study, 17.8% of all recordings of early signs and 9% of late signs were judged by nurses...
- 6
- 494
- 0
Từ khóa: Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ