Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc

... AccessConcomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa- treated subjects at early times after repeated local cytokine treatmentsEleonora ... Concomita nt detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa- treated subjects at early times after repeated local cytokine treatments. ... Consistency of IFNa signature in d ifferent in vivo and in vitro settings. Heatmap of the 74 cDNA consistently up-regulated by IFNa in any of the in vivo and in vitro settings analyzed. The lists of IFNa- up-regulated...

báo cáo hóa học: " Cyclooxygenase-2 mediates microglial activation and secondary dopaminergic cell death in the mouse MPTP model of Parkinson''''s disease" pptx

... of oxidative and inflammatory insults. The positive feed back loop may continue until the additional neu-ronal death (~50% of the remaining of the initial death) com-bined with the initial ... and denser stain. B: MPTP treatment leads to a significant increase in the number of activated microglia in the WT relative to the HT and KO mice. Activated microglia in the SNpc were bilaterally ... activation, degeneration of TH-positive cells, and loss of coordination.Conclusion: These results indicate that reducing COX-2 activity can mitigate the secondary and progressive loss of dopaminergic...

Báo cáo sinh học: " Virus detection and identification using random multiplex (RT)-PCR with 3''''-locked random primers" docx

... coordinated the distribution of infor-mation and infected materials and the analyses of the dataReal-Time PCR Quantitation of Adenovirus Type 17 DNA TemplateFigure 5Real-Time PCR Quantitation of ... (lower band). Although the addition of RNAse alone has little effect on the β-actin mRNA, the combination of DNAse and RNAse completely eliminatesβ-actin mRNA. We postulate that this finding may ... compar-ing infected cells with non-infected cells [9]. The otherstrategy is known as Sequence-Independent, Single-Primer Amplification (SISPA) and involves the directionalligation of a linker/adaptor...

Báo cáo sinh học: " Respiratory syncytial virus-induced acute and chronic airway disease is independent of genetic background: An experimental murine model" pot

... 5). The CC chemokines, RANTES and MIP-1α, and the CXCchemokine MIG were also elevated in mice of both strainsshortly after RSV inoculation. The biphasic production of RANTES, MIP-1α and MIG after ... strains [8] but therewas no information on whether the different genetic back-grounds influence the long term outcome of RSV infectionincluding chronic inflammation and persistent AO and AHR. ... conducted in mice with primary RSV infec-tion, demonstrated that IFN-γ, a Th1 cytokine, waselevated after RSV infection in both strains. IFN-γ wasincreased in the respiratory tract of children...

Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot

... A, and 10 min isocratic gradientwith 2%. Fractions were collected in 5 minute intervals for the first 20 min and then in 2 min intervals for 60 min. The final 7 min was collected in one final ... resus-pending the insoluble pellet from the virion fractionation in 0.4% sodium deoxycholate and 10 mM Tris, pH = 9.0, and incubating at 56°C for 10 min. The sample was fil-tered and injected onto the ... accelerating the precursor ions to 8 keV,selecting with the timed gate set to 3 Da, and performing the collision induced dissociation (CID) at 1 keV. The gaspressure (air) in the CID cell was at...

Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt

... EMSAs.N-terminal sequencing of the 70-kDa protein was inhibitedby modiﬁcation, consistent with previous reports showingthat the amino terminus of the human Ku-70 protein isblocked and therefore inaccessible ... examined the effect of the Ku proteins on the transcriptional regulation of the WD gene. First, weoverexpressed Ku protein in HepG2 cells, and the resultsindicated that overexpression of Ku ... binding reaction was carried out for 30 min at 25 °C. After elinimating by centrifugation denatured pro-teins generated during the binding reaction, the mixture wascombined with 20 lL avidin–agarose...

báo cáo hóa học:" Survivin gene levels in the peripheral blood of patients with gastric cancer independently predict survival" doc

... evaluat-ing gene expression, have been used in the detection of tumor markers that indicate the presence of CTC in the blood [8]. In the current analysis, we aimed to profiling the periph-eral blood ... the currentTNM staging system. The validation of these findings in larger prospective and multicentric seriesmight lead to the implementation of this biomarker in the routine clinical setting ... patients'OS. The hazard ratio (HR) associated with Survivin levelsindicates the increase in risk of death for 100 fold increase in Survivin expression (Table 3).Since only these four independent...

báo cáo sinh học:" Human resources for health planning and management in the Eastern Mediterranean region: facts, gaps and forward thinking for research and policy" pot

... confirm the importance of HRH and other determinants in affecting health outcomes. Animplication of our results is that investing and pouring in more money to increase the number of physicians and nurses ... 2004,364:1603-1609.8. Robinson JJ, Wharrad H: The relationship between attendance at birth and maternal mortality rates: an exploration of United Nations' data sets including the ratios of physicians and nurses ... the findings of other studies, which note that the presence of appropriate medical personnel to performsuitable medical interventions is significant for preventing the death of mothers and infants...

báo cáo sinh học:" Workforce analysis using data mining and linear regression to understand HIV/AIDS prevalence patterns" pdf

... performed the data mining. EAM performed the multiple regressionanalysis. The generation of the idea and writing of the paper was a three way effort in drafting and revising the final copy. ... differentyears and different sources of information, which mayhave resulted in missing some of the dynamic nature of the change in the variables used in the combined database. Finally, our study ... Development and Population Assistance (UNFDPA) 2000, the International Council of Nurses "Nursing in the World", and the WHO/UNAIDS database.Results: The main factors in understanding HIV/AIDS...

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