Báo cáo sinh học: "High throughput RNAi assay optimization using adherent cell cytometry" pot

... vascular cells using small interfering RNAs. J Am Coll Surg 2007, 204:399-408. doi:10.1186/1479-5876-9-48 Cite this article as: Nabzdyk et al.: High throughput RNAi assay optimization using adherent cell ... This technology can accelerate in vitro cell assays and thus save costs. Keywords: RNAi vascular, adherent cell cytometry, in vitro assay, high throughput Backg...

Ngày tải lên: 18/06/2014, 19:20

... vascular cells using small interfering RNAs. J Am Coll Surg 2007, 204:399-408. doi:10.1186/1479-5876-9-48 Cite this article as: Nabzdyk et al.: High throughput RNAi assay optimization using adherent cell ... This technology can accelerate in vitro cell assays and thus save costs. Keywords: RNAi vascular, adherent cell cytometry, in vitro assay, high throughput Backg...

Ngày tải lên: 20/06/2014, 03:20

... A+PSA assay did not interfere with the quantification of individual autoAb, autoAb against the prototype NY-ESO-1:1-40 epitope using the multiplex A+PSA assay were compared with those measured using ... the XAGE-1b epitope were observed using the seroMAP- based approach over ELIS A. The multiplex A+PSA assay quantifies autoAb and total PSA in one reaction To develop a multiplex a...

Ngày tải lên: 18/06/2014, 19:20

... a cell has been uncovered. Parallel efforts in either yeast [10,11] or D. melanogaster [12,13] show little overlap in their data sets, and approaches based on using small fragments in the assay ... high- throughput (HT) usage. This stage is made possible by advances in some combination of automation, minia- turization, reduction in reagent costs and further refinements of the approach....

Ngày tải lên: 23/03/2014, 15:21

... deployed on a 20 CPU Beowulf cluster using AMD Athlon™ processors running Linux operating system. For production deployment we used Message Driven Beans (MDBs )using IBM Websphere Application Server™ ... Buntrock@mayo.edu duffp@mayo.edu Abstract This paper presents the results of the de- velopment of a high throughput, real time modularized text analysis and information retrieval...

Ngày tải lên: 31/03/2014, 03:20

... The pathologists recorded whether tumor or stromal cells exp ressed ERCC1 . The staining intensity was graded on a scale of 0-3, using adjacent nonmalignant cells as a reference (intensity 2). Five images ... squamous cell carcinomas of the head and neck. Cancer Res 1999, 59:1935-1940. 35. Huang SM, Harari PM: Modulation of radiation response after epidermal growth factor receptor blockad...

Ngày tải lên: 18/06/2014, 19:20

... lines in cellular proliferation assays and from cell cycle analyses. Cell lines were classified into one of three categories based on the time when the majority of cells contained sub-2N DNA (cell ... observed 6 of the 7 T- ALL cell lines with high chromosome number also had Figure 1 Response profile of G SK1070916 for he matological cell lines using cell cycle analysis and cell...

Ngày tải lên: 18/06/2014, 22:20

... virus-infected or trans- ducted cells. 1. Protein marker; 2. vAc-Proie1-EGFP infected SF9 cells; 3. vAc-Pro427-EGFP infected SF9 cells; 4. vAc-Proie1- EGFP transduced shrimp primary cells; 5. vAc-Pro427-EGFP ... shrimp cells. A vAc-Pro ie1 -EGFP vAc-Pro 427 -EGFP EGFP cDNA EGFP cDNA P ie1 P 427 B C SF9 cells infected with vAc-Pro ie1 -EGFP Primary cells transduced by vAc-Pro ie1 -EGFP Primary...

Ngày tải lên: 19/06/2014, 08:20

... districts of Tamil Nadu State. Cell line Porcine Kidney (PS) cells (supply No 3109 A) obtained from National Center for Cell Sciences, Pune, were used in the study. Cells were grown in plastic tissue ... served as positive controls for the shell vial assay in this study. Procedure for Shell vial assay 1 ml of the porcine kidney cells suspension (4 × 10 5 cells/ ml) in growth medium w...

Ngày tải lên: 19/06/2014, 08:20

... texts using finite state transducers. The texts are then part- of-speech (POS) tagged and separated into origi- nal form words z. Original forms of English words are determined by 80 rules using ... step adjusts ASM using the AM constructed by the above operations. The sentence pairs that have at least ANC word correspondences are deter- mined to be new anchors. By using the...

Ngày tải lên: 31/03/2014, 06:20