... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal ... Mn-reconstitutedApeSOD, the metal ion was five-coordinate in trigonalbipyramidal geometry (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ... protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles...