báo cáo hóa học: " Telephone reliability of the Frenchay Activity Index and EQ-5D amongst older adults" pptx
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... 3325). For the construction of a tagged version of CCR5, an XhoI restriction site was introduced upstream of the start codon of CCR5 and an ApaI site upstream of the stop codon by PCR and expanded ... inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of signalling is a down-mod...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx
... CENP-B and the unmethylated CENP-B box DNA at sites 1 and 3. The pink dotted line in the right panel indicates the hydrogen bond between the side chain NH 2 group of Arg125 and the N7 atom of guanine. Y. ... and the N7 atom of guanine 16 by the CpG methylation may be a reason for the reduced specificity of CENP-B to the CENP-B box sequence. What is the fun...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo y học: " Experimental ablation of the pancreas with high intensity focused ultrasound (HIFU) in a porcine model"
... During the therapeutic process, real-time estimation of the therapeutic effect was carried out by the computer system through the graphic changes in the target field and the hyperecho of the ... transducer and the skin in order to well locate the target region. The real-time US imaging device was used to locate the head of pancreas as the pre-designed t...
Ngày tải lên: 25/10/2012, 11:18
Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in fish pdf
... molecu- lar activity of Sry is translated into cellular structure and organ morphology. Interestingly, Dmrt1, the ancestor of Dmrt1bY, is one of the downstream effec- tors of Sry in the male pathway. In ... birds, mammals and corals is the dmrt gene family [5]. The prototype members of this group of factors are the Drosophila doublesex (dsx) and Caenor- habditi...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... ligands in the Mn-reconstituted enzyme and an additional water oxy- gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial plane (Fig. 3B). The metal ... Mn-reconstituted ApeSOD, the metal ion was five-coordinate in trigonal bipyramidal geometry (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... this RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We consider the first option unlikely. Under these ... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from the R1E...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... monomer and dimer, and that Thr binding shifts the equilibrium to dimer formation. In the absence of Thr, an outward shift of b-strands near the Thr-binding site (site 1) and a concomitant loss of the ... regulatory mechanism of Thr and the struc- tural features responsible for the high thermostability of TtAKb. Results and Discussion Model quality The crysta...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... expression and this enhancement was dependent on the RNA-dependent RNA polymerase activity of NS5B, but NS3-4A significantly inhibited the production of IFN-b induced by the combination of Core and NS5B. ... that the activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtained further evidence by examining the status of the...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... erva- tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3 ... at the bottom of the subsite is conserved in ervatamins. Compared to a Ser in the equivalent position of papain, the role of Leu here is to restrict the s...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... between the two steps of the TES reaction The substrate guanosine to be excised (G 1 ) and its 2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... the integrity of the bound species during gel electrophoresis, the gel and the running buffer were made of H10Mg buffer and were prewarmed to 44 °C befo...
Ngày tải lên: 18/02/2014, 18:20