... in a 37°C incubator under 5% CO2.The cells were transferred to the serum free media (AIM-V,Gibco Ltd., Carlsbad, California, USA) for up to 48 hoursprior to the experiment to remove any conflicting ... bioreac-tors. The glass bioreactors were specifically designed to collect the gaseous headspace above aqueous cultures (seeFigure 1) [19]. The bioreactor consisted of two glass halvesjoined together ... bio-reactors simultaneously. A needle valve (Swagelok, Solon,OH) and flowmeter (Dwyer Instruments Inc. MichiganCity, Indiana, USA) was used to adjust the net flow to thebioreactors to 2500...