Báo cáo sinh học: "Construction and characterization of an infectious clone of coxsackievirus A16" docx

... without and with the EGFP cassete (lanes 1 and 2, respectively); DNA amplification of plasmid T3 and the recombinant one (lanes 3 and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic ... independent series of serial passages (at MOI of 0.02); P1 and P2 were analyzed by RT-PCR and flow citometry at passages 5 and 10 and are represented in all pa...

Ngày tải lên: 18/06/2014, 18:20

... produced from the cDNA clone. The resultant transcripts were used to transfect RD cells. At 72 h post-transfection, cells and supernatants were harvested and analyzed by microscopy and biochemical ... and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16. Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Is...

Ngày tải lên: 18/06/2014, 18:20

... systematic analysis of pub- lication and citation data from tens of thousands of articles can capture important features of active fields of scientific research, in this case in both cancer and cardiac ... 2000, Sobin 2002) and the cardiac plot (Schiller 1 989, Chobanian 20 03, Lang 2005, Mancia 2007). The prominence of such guidelines demonstrates the importance of standa...

Ngày tải lên: 18/06/2014, 19:20

... these clones, 24 clones were from animal 225, and 19 clones from animal 321. A por- tion of gp41 was sequenced in each clone to insure the identity of the clones and to determine if any of the clones contained ... motor, and neurophysiological impairments [11-15]. The similarities between HIV- and SIV-induced neurological disease in humans and macaques, in light of the eth...

Ngày tải lên: 19/06/2014, 08:20

... [22]. Parameters of oxidative damage and antioxidant defenses in liver and brain homogenates Lipid peroxidation Malondianldehyde (MDA) was used as an index of lipid peroxidation [27] in liver and brain ... hormones and serum total antioxidant status Consistent with previous studies [4], old anima ls showed a reduction in IGF-I and testosterone levels and a statisti- cally si...

Ngày tải lên: 18/06/2014, 19:20

... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol. ... A + ,B + and O + ), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity. P I and P II promoted hem- agglutinatio...

Ngày tải lên: 18/02/2014, 16:20

... Kalkkinen 2 , Johanna Buchert 1 and Kristiina Kruus 1 1 VTT Technical Research Centre of Finland, Espoo, Finland 2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of ... microorganisms, and in mammals, inverte- brates and plants. Tyrosinase is a mono-oxygenase and a bifunctional enzyme that catalyzes the o-hydroxyla- tion of monophe...

Ngày tải lên: 19/02/2014, 06:20

... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control ... 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nuc- leus, and Fig. 7C, F, K and N are the merged images of A and B, D an...

Ngày tải lên: 19/02/2014, 07:20

... of HSF, whereas clone C2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids. By using 5¢ -and3 ¢-RACE, the full-length cDNAs of clones C1 and C2 without ... 7A, lane 6 and Fig. 7B, lane 3). Given that the two HSF1 isoforms form both homo- and heterotrimers, there are four potential assemblies of HSF1 trimer, namely, two homotrim...

Ngày tải lên: 19/02/2014, 12:20

... cause of these differences is not known. K m and k cat measurements of hevamine and mutants Comparison of the steady-state kinetic parameters of hevamine and the Tyr183Phe and Asp125Asn mutants shows ... is one of the most abundant polymers in nature. It is a major component of the cell wall of yeast and other fungi, and the exoskeleton of arthropods. Although chit...

Ngày tải lên: 22/02/2014, 04:20