Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf

... Access Methodology Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses Sarah Watson 1 , ... Ref- erence gene selection for quantitative real- time PCR analy- sis in virus infected cells: SARS corona virus, Yellow fe...

Ngày tải lên: 18/06/2014, 18:20

... Access Methodology Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses Sarah Watson 1 , ... researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments. Back...

Ngày tải lên: 20/06/2014, 01:20

... infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral ... cells. Background Quantitative real- time PCR (QPCR) has become the favoured tool in mRNA expression analysis and also in virus diagnostics [1]. Real- time...

Ngày tải lên: 18/06/2014, 22:20

... infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral ... cells. Background Quantitative real- time PCR (QPCR) has become the favoured tool in mRNA expression analysis and also in virus diagnostics [1]. Real- time...

Ngày tải lên: 20/06/2014, 04:20

... al., Real- time detection of DNA hybridization and melting on oligonu- cleotide arrays by using optical wave guides,” Proceedings of the National Academy of Sciences of the United States of America, vol. ... estimation of the parameters of the process which generated the described experimental data. We run 5000 iterations of the algorithm with M = 5andK = 1500, and ave...

Ngày tải lên: 21/06/2014, 08:20

... health workforce. Human Resources for Health 2004, 2(13). 7. Gupta N, Diallo K, Zurn P, Dal Poz MR: Assessing human resources for health: what can be learned from labour force surveys? Human Resources for ... quantity of Fulton et al . Human Resources for Health 2011, 9:1 http://www .human- resources-health.com/content/9/1/1 Page 2 of 11 efficient skill mix will vary acros...

Ngày tải lên: 18/06/2014, 17:20

... MN). Immunohistochemical analysis of p65 nuclear translocation B16M cells (1 × 10 4 cells/ well)weregrownon8μm- chamber glass slides. Cells were serum-starved for 24 h and treated with IL-18 (100 ng/ml) for 30, 60 and 120 ... to be done for a more complete understanding of its mechanisms of action and ther efo re, for a better assessment of its anti- tumor effic...

Ngày tải lên: 18/06/2014, 19:20

... 100 μL PBS for 10’ at 4°C. Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before perme abilization for intracellular staining. To permeabi- lize the cells, pellet ... positive for detection of CTCs if at least one sample resulted to be positive for presence of CTCs. Table 3 Comparison of EpCAM and cytokeratin (CK) expression profile...

Ngày tải lên: 18/06/2014, 19:20

... devices. Real- time oral malodour measurements After demonstrating the ability of the RIfS system for detection of H 2 S, the performance of our device was examined for oral malodour analysis ... malodour with major presence of H 2 S that arises from bacterial metabolism of amino acids and proteins is another example of biomedical determination of H 2 S that...

Ngày tải lên: 18/06/2014, 22:20

... born before time, sometimes with tragic consequences. Uterine electromyography has been a research subject for many years since the 1950s. It has been proven to be of interest for pregnancy and ... P. Morucci, H. Grandjean, and R. Favretto, “External recording and processing of fast electrical activity of the uterus in human parturition,” Medical and Biological Engineer...

Ngày tải lên: 21/06/2014, 16:20