Báo cáo sinh học: " Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages" docx

... for citation purposes) Virology Journal Open Access Research Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes ... mutagenesis of the V3 V5 regions and binding affinity of gp120 to CCR5 and/ or gp120 incorporation into chimeric virions might pinpoin...

Ngày tải lên: 18/06/2014, 18:20

... for citation purposes) Virology Journal Open Access Research Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes ... biological properties of HIV-1 subtype C, including cellular tropism, virus entry, replication efficiency and cytopathic effects, we...

Ngày tải lên: 20/06/2014, 01:20

... RT-PCR using forward 5'- CCCCATTTTCCTCTA GCGACTG-3' and reverse 5'-CGGCCGCATGGT- TCTCGCCAAT-3' primers (regions corresponding to 15,386 to 15,846 bp of the PRRSV VR-2332) and ... for 30 min. To demonstrate the correlation between CD151 presence and susceptibility to PRRSV infection, RT-PCR was carried out using CD151 specific primers 5'-CCTACCTGGCCACA...

Ngày tải lên: 18/06/2014, 18:20

... R5- TM-F, CATGGAGATGTTAAATTTAGATTATAAATTAGGAGTAGG AGCATTGATAGTAGCACTAATCATAGCAATAGTCGTGT- GGACCATAGTATATATAGAATAGG and R5-TM-R, AATT CCTATTCTATATATACTATGGTCCACACGACTATT- GCTATGATTAGTGCTA CTATCAATGCTCCTACTC- CTAATTTATAATCTAAATTTAACATCTC. The cytoplasmic domains ... NL-TM-R, AATTCCTATTCTATGATTACTATGGACCACACAGCTATT GCTATTATTATTGCTACTACTAATGCTACTATTGCTAC- TATTATAGGTTGCATCTC; for the R5 vpu TM r...

Ngày tải lên: 18/06/2014, 18:20

... (5’-GTCGAC(Sal I) CACCATGGAGGCCGTG- GAGACCGG-3’ )andhNIS-3(5’-TTAATTAA(Pac I) TCAGAGGTTTGTCTCCTGCTGGTCTCGA-3’ ). The PCR product was gel purified, and cloned into the pCR- Blunt II-TOPO vector using ... triplicate. GLV-1h153 was cytolytic against PANC-1 cells in vitro To investigate whether expression of hNIS would affect cytolytic activity of VACV in cell cultures, PANC-1 cells w...

Ngày tải lên: 18/06/2014, 19:20

... negative cga gac ctc ccg ggg cac tcg caa gca ccc HCV 3 negative acg cag aaa gcg tct agc cat ggc gtt agt HCV 4 negative tcc cgg ggc act cgc aag cac cct atc agg HutLA2 positive ggg ccg ggc atg aga cac ... positive cat gat gca cgc tct acg aga c HCV 10.1 positive ctg tga gga act act gtc ttc acg cag HCV 10.2 positive cac tcg caa cca ccc tat cag HCV 1 negative act gtc ttc acg cag aag cgt cta gcc...

Ngày tải lên: 19/06/2014, 08:20

... attributed in part to an increase in circulating levels of cholecystokinin. Similarly, in rats fed a hy- percholesterolemic diet, ingestion of the alpha subunit of the soy 7S globulin (conglycinin) ... protein and carbohydrate sources, namely, soy protein isolate (SPI)-cornstarch, SPI-sucrose, cod protein (COD)-cornstarch, COD-sucrose, casein- (CAS)-cornstarch, CAS-sucrose...

Ngày tải lên: 31/10/2012, 14:59

... (bp) CD163 (XM_053094.2) Sense: AGCTGGGCTGTGCAGACAACG Antisense: TGAATGACCCCCGAGGATTTCAGC 736 CYP1A1 (X00469) Sense: CTGGTTCTGGATACCCAGCTG Antisense: CCTAGGGTTGGTTACCAGG 331 CYP1A2 (X01031) Sense: CAGTCACAACAGCCATCTTC Antisense: CCACTGCTTCTCATCATGGT 302 CYP2B1 (XM_342078) Sense: TTGTTTGGTGCTGGGACAGAG Antisense: GGCTAGGCCCTCTCCTGCACA 443 CYP2E1 (M20131) Sense: AAACTTCATGAAGAAATTGAC Antisense:...

Ngày tải lên: 14/02/2014, 18:20

... (7) and the release of pro -in ammatory cytokines such as IL-8 (8). These cytokines can alter the secretion of mucus and induce changes in gastric acid secretion and homeostasis. They also attract ... Shp2. Instead, CagA PY can inhibit Src activity both by direct interaction of both proteins or by binding of CagA PY to Csk, a negative regulator of Src [6,7]. Because Src is...

Ngày tải lên: 14/02/2014, 19:20

... oligonu- cleotides and nuclear extracts from SP1 cells. The nucleo- tide sequences of putative CERK-PPRE, including the sense 5¢-CTCTCCAGGCCACAGGCCAGAGCGG-3¢ and anti-sense 5¢-GAGAGGTCCGGTGTCCGGTCTCGCC-3¢ sequences, ... Cer is incorporated, hydrolyzed to sphingosine and then recycled into intracellular Cer itself, and that accumu- lation of Cer contributes to the induction of...

Ngày tải lên: 18/02/2014, 18:20