Báo cáo sinh học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" potx

... purposes) Virology Journal Open Access Research In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter Agustín Luz-Madrigal 1 , ... Furthermore, only two reports show to this date in vivo transcriptional gene targeting by recombinant baculovirus. In this study, we produced a rec...

Ngày tải lên: 18/06/2014, 18:20

... purposes) Virology Journal Open Access Research In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter Agustín Luz-Madrigal 1 , ... containing the 1-kb region from the human flt-1 promoter is able to drive transcriptional gene targeting in vitro and in vivo in the retin...

Ngày tải lên: 20/06/2014, 01:20

... likely that the large increase in virus over a 24 hour cycle observed in vitro also occurs in vivo, resulting in a signifi- cant daily increase in the titer of Hz-2V in these experi- mentally infected ... participated in the design of the study, carried out the work with the insects, coordinated the project and assisted in the molecular analysis and drafting o...

Ngày tải lên: 18/06/2014, 22:20

... from these compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5]. We show here that haem analogues are indeed taken up and can be incorporated into ... that the metal ion of the porphyrin is removed during transport of the porphy- rin from the medium to the catalase protein in the cytoplasm. Alternatively, but less likely,...

Ngày tải lên: 06/03/2014, 11:20

... oyvl-dsRNA into the target organ. The DIG-labelled oyvl-dsRNA has been observed in a large part of the gonad around the injection point, showing the efficiency of the administration of the dsRNA into the ... oyster tissues. The DIG-labelled dsRNA developed in the present study represents an important technical advance for examining the first crucial step in succe...

Ngày tải lên: 07/03/2014, 00:20

... that the RAR binding was almost undetectable in vitamin A-deficiency when compared to controls, while in acute hypervitaminosis the binding was significantly higher. These changes in the RAR–DNA binding ... found. In lung, an increase in the expression of c-Jun produced a significant increase in cyclin D1 expression. These results may explain, at least in part, the conflic...

Ngày tải lên: 08/03/2014, 08:20

... membrane properties. As in past reports, the j oint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of d irect effects, ... in the mutants [18]. On the other hand, this activation showed particular characteristics, namely, it is nonessential, and involves changes in the V max of the protein...

Ngày tải lên: 08/03/2014, 22:20

... more pronounced. Moreover, in brain, the natural inhibitor of calpain, calpastatin, was preferentially converted into still active 15 kDa fragments (Table 1), whereas, in aorta, the inhibitor was predominantly inactivated. ... promoting NO production also induce calpain activation. Thus, the formation of NOS–HSP90 complexes may provide a new insight into the understanding of...

Ngày tải lên: 30/03/2014, 04:20

... transglutaminase in sperm chromatin We next examined whether nuclear transglutaminase is present in the chromatin fraction by carrying out an immunoblot analysis using anti-(nuclear transglutaminase) Ig ... transglutaminase by an increase in the intracellular concentration of Ca 2+ .To our knowledge, this is the first demonstration of intracellular transglutaminase-catalyzed pr...

Ngày tải lên: 31/03/2014, 07:20

... [3,5-7]. In spite of all, clinical trials using gene or protein therapy with VEGF isoforms for treatment of myocardial or peripheral ischemia have been somewhat disappointing indicating the needs ... analysis in the ischemic hindlimb as well as by histology in wounds and Matrigel plugs. Our findings show the proangiogenic properties of QK, suggesting that also in vivo this p...

Ngày tải lên: 18/06/2014, 15:20