Báo cáo sinh học: " Oligomerization of the human immunodeficiency virus type 1 (HIV-1) Vpu protein – a genetic, biochemical and biophysical analysis" ppt

... R5- TM-F, CATGGAGATGTTAAATTTAGATTATAAATTAGGAGTAGG AGCATTGATAGTAGCACTAATCATAGCAATAGTCGTGT- GGACCATAGTATATATAGAATAGG and R5-TM-R, AATT CCTATTCTATATATACTATGGTCCACACGACTATT- GCTATGATTAGTGCTA CTATCAATGCTCCTACTC- CTAATTTATAATCTAAATTTAACATCTC. ... GAATTCACTACAGATCATCAATATCCCAAG; for the R5 vpu gene, Vpu- R5-F, GGATCCATGTTAAATTTA- GATTATAAATTAGGAGTA GG and Vpu- R5-R, GAAT- TCATTACAAATCATTAA...

Ngày tải lên: 18/06/2014, 18:20

... paper and was critical to study design and completion. All authors have read and approved the final manuscript. Author disclosure statement Nanhai G. Chen, Qian Zhang, Yong A. Yu, and Aladar A. ... non-invasive imaging of virotherapy alters the replication and oncolytic capability of a novel vaccinia virus, GLV-1h153. Methods: GLV-1h153 was modified from parental vaccin...

Ngày tải lên: 18/06/2014, 19:20

... 8 and 9) and rabbit anti-MUC5B (lanes 10 , 11 and 12 ) polyclonal antibodies. Membranes were then incubated for 1 h with HRPO linked goat anti-mouse and goat anti-rabbit secondary antibodies and ... replication was measured by a qualitative p24 antigen assay. Let- ters a, b, c and d indicate the anti-HIV -1 activity of each sample in a serial tenfold dilution of...

Ngày tải lên: 20/06/2014, 01:20

... Rad 51 L1 and L2 loops in DNA binding Yusuke Matsuo 1 , Isao Sakane 2 , Yoshimasa Takizawa 1 , Masayuki Takahashi 3 and Hitoshi Kurumizaka 1, 2 1 Graduate School of Science and Engineering, Waseda ... under- standing the reaction mechanism and the regulation of homologous recombination. So far, the crystal structures of bacterial RecA, ar- chaeal Rad 51 (RadA), ye...

Ngày tải lên: 19/02/2014, 06:20

... B2-linker-ATTGTAACGGCTATATCTACTGG ALR-c36SU3¢ B2-linker-GGCAAGAAGCTCGAAATAACC ALR-c63SU3¢ B2-linker-CCAATAGCTGGCCAAGAACC ALR-c96SU3¢ B2-linker-ATTCATACCGAAAAGACC C ALR2-HIS-f ATTTTTATGAGAAAACGTGAAAAAACTTC GTAATGTCGTCCTTATCGTACGCTGCA GGTCGAC ALR2-HIS-r ... AAA CTGCAG GATTGTAACGGCTATAT CTAC E Alr1-rtp CAGGGTATGGATGAAACGGTTGC Alr1-rtm TGATCCCGAAGTGGAAGTAGAGC Alr2-rtp TTAAGTTCTAATGCGAGGCCATCC Alr...

Ngày tải lên: 19/02/2014, 06:20

... CGGGTACCTACTCAGGAAGCATGC AAGT (sense strand of the sequence from )67 to )47) for phLP4; CGGGTACCACAGAAAGTGAAAGCA (sense strand of the sequence from )33 to )18 ) for phLP5; CGACGCGTTGACTGAAGGGGAATTTACTTT (antisense ... site) and 5 ¢-CGACGCGTTGACTGAAGG GGAATTTACTTT-3¢ (antisense sequence of mutant Ets site) were used. For the construction of the double mutant plasmid, 5¢-GGGGT...

Ngày tải lên: 23/03/2014, 13:20

... more than 50% of the cell specific associated radioligand was internalized (Fig. 2). The internalization reached a plateau after 10 –2 0 min, with a maximal radio- ligand internalization of 69%. ... presence of 10 )7 M unlabelled CRF, was subtracted and the radio- activity internalized was expressed as a percentage of the sum of the surface radioactivity and...

Ngày tải lên: 30/03/2014, 15:20

... lack of a sustainable financial policy, inadequate infrastructure, under-trained faculty members in some areas, poor instructional materials and equip- ment and lack of a formal evaluation and accreditation mechanism, ... Egypt and 3 Suez Canal University, Egypt Email: Galal Abdel-Hamid Abdellah - galalabdellah@mailer.eun.eg; Salah El-Din Mohamed Fahmy Taher - sftaher@yahoo.c...

Ngày tải lên: 18/06/2014, 17:20

... Int viral protein ( #11 15 )/Int hostprotein ( #11 15 ) and in similar manner for 3 dpi medium. 3 Purification factor was estimated as the viral to host ratio in #11 15 divided by the similar ratio ... 11 15 , concentrated the samples by centrifugation, embedded the pellets in gela- tine and performed EM-analyses. The analyses of the gra- dient peak fractions 11 15 sho...

Ngày tải lên: 18/06/2014, 18:20