... substrate binding, catalyticefficiency (kcat⁄ KM) decreased in direct relation to < /b> the< /b> kcatvalues (Table 2).These results < /b> show that the < /b> contribution of < /b> eachCK domain to < /b> catalysis depends ... physio-logical roles of < /b> the < /b> CK reaction are greatly facilitatedby the < /b> presence of < /b> three nuclear gene families, eachtargeted to < /b> and localized in speci c intracellularcompartments – cytoplasmic (CyCK), ... mutateddomains were constructed: D1 S D2 D3, D1 D2S D3 , D1 D 2D3 S, D1 D2S D3 S ,D1 S D2 D3 S ,D1 S D2 S D3 and D1 S D2 S D3 S(where the < /b> subscript S corresponds to < /b> the< /b> C fi S mutant and no subscript...