Báo cáo hóa học: " FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy" docx

... distribution, and reproduc- tion in any medium, provided the original work is properly cited. Research FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy Laura Fiammenghi †1 , ... this article as: Fiammenghi et al., FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy...

Ngày tải lên: 18/06/2014, 16:20

... of both brain tumors and peripheral tumors is much larger. In the case of brain tumors, inter-endothelial cell gaps have been reported to range between 100 nm and 3000 nm in diameter[10,13] and in the ... were stereotacti- cally inoculated with 10 5 RG-2 glioma cells in 5 μL of ster- ile PBS. In each location, the cells were injected over 8 minutes, using a 10 μL Hamilton syringe...

Ngày tải lên: 18/06/2014, 15:20

... TFIIB, consisting of 316 amino acid residues, is comprised of a N-terminal domain (NTD) that contains the Zn 2+ ribbon motif, and a C-terminal core domain (CTD) possessing two repeats of the cyclin fold ... functionally distinct domains are connected via a highly conserved linker containing several charged residues, hereafter termed a charged cluster domain (CCD), critical for maintaining TFI...

Ngày tải lên: 07/03/2014, 15:20

... Science and Engineering, Tsinghua University, Beijing, PR China, 3 Department of Anatomical and Cellular Pathology, Chinese University of Hong Kong, Hong Kong, PR China, 4 Li Ka Shing Institute ... Zhongshanjinqiao, China) in PBS for 10 minutes. Cells were washed with 1% NGS in PBS and incubated overnight at 4°C with the fol- lowing primary antibodies diluted in PBS containing 1% NG...

Ngày tải lên: 18/06/2014, 15:20

... gastric cancer cell line, we found some differences in the protein patterns but overall, these changes were small: Seven pro- teins (with two variants) were clearly defined in the gastric carcinoma cell line ... fixed in 50% ethanol, and 10% acetic acid for 12 hours. Silver staining Proteins were visualized using the silver staining method employing a modification of the method of H...

Ngày tải lên: 18/06/2014, 15:20

... 1.27 fibronectin 1 FN1 2.10E-07 2.43 5.45E-05 3.77 serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 SERPINE1 1.54E-09 5.69 0.000334 10.83 TGFB-induced ... and ZYX). Integrins have been shown to mediate the activation of TGFβ [41] and TGFβ is known to regulate the expression of cell adhe- sion molecules including integrins [42,43]....

Ngày tải lên: 18/06/2014, 15:20

... senescent cells increased in Cell proliferation kinetics of vascular cell types obtained from human umbilical cords (UCs)Figure 1 Cell proliferation kinetics of vascular cell types obtained from human ... in the corresponding study. Aberrant multinucleated cells were excluded from the distribution analysis. The binding and internalization of Ac-LDL was determined by incubating cel...

Ngày tải lên: 18/06/2014, 15:20

... transcription regula- tion in erythroid cells. Since miRNA represent a new class of transcription regulators in eukaryotic cells, human cir- culating erythroid cells were used to determine whether fetal-to-adult ... let-7 in adult erythroid cells. We speculate that let-7 or other differentially expressed miRNA are involved in the hemoglobin switching phenomenon. Alternatively, the inc...

Ngày tải lên: 18/06/2014, 15:20

... leading to a massive production of proin- flammatory cytokines including interleukin-2 and inter- feron-g [11], resulting in extensive proliferation of T cells bearing specific Vb subgroups[11].Asaresult,it is ... presenting cells (APCs) [1]. During thymocyte development, the genes encoding the b chain undergo somatic recombinatio n of va riable (V), diversity (D), joining (J), and constant...

Ngày tải lên: 18/06/2014, 16:20

... rapamycin, single agent aspara ginase, combination asparag inase plus rapamycin, single agent vincristine, combination vincristine plus rapamycin, single agent sunitinib, combination sunitinib plus ... Bevacizumab) Untreated Rapamycin Vincristine Combination Vincristine plus Rapamycin Asparaginase Combination Asparaginase plus Rapamycin Sunitinib Combination Sunitinib plus Rapamycin Bevacizum...

Ngày tải lên: 18/06/2014, 16:20