... purposes)
Journal of Translational Medicine
Open Access
Commentary
Translating molecular medicine into clinical tools: doomed to fail by
neglecting basic preanalytical principles
Klaus Jung*
1,2
, ... tissue inhibitors (TIMPs), we would
like to draw attention on the preanalytical impact of
blood collection/handling methods in order to limit tech-
nical pitfal...
... intron regions, mito-
chondrial 12S rRNA (nt611 to nt2007), and tRNA
ser(UCN)
(nt7148 to nt8095) genes were amplified by PCR fol-
lowed by sequencing using the Big Dye sequencing proto-
col in all ... GJB6 was
analyzed by PCR [15,16]. A positive control (provided by
Balin Wu, Department of Laboratory Medicine, Children's
Hospital Boston and Harvard Medical School, Boston,
MA...
... places chosen
by the participants (coffeehouses, neighborhood circles).
Interview questions were translated into Portuguese by
professional translators and translated back into German
by the accompanying ... which are grouped into 7
component scores, each weighted equally on a 0 to 3
scale. The 7 component scores are then totalled to provide
a global PSQI score, which has a ra...
... Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan,
2
Institute of Clinical Medicine and Research, The Jikei University School of Medicine, Tokyo, ... Institute of DNA
Medicine, The Jikei University School of Medicine, Tokyo, Japan,
4
Clinical Data Bank, Institute of DNA Medicine, The Jikei University School of
Medicine, Tokyo, Ja...
... amenable to standardization and
automation for the monitoring of cellular immune
responses in WB, simple enough to be performed, at least
in its initial steps, by personnel with basic laboratory
training, ... quantification on
whole blood to assess allergic response. Cytokine 2005,
31:375-381.
14. Stordeur P, Zhou L, Byl B, Brohet F, Burny W, De GD, van der PT,
Goldman M: Immune mon...
... (Qiagen). Total RNA was quantified by
RiboGreen (Invitrogen, Carlsbad, CA). For determination
of drug levels, compounds were extracted from EDTA
plasma into 1:1 acetonitrile:water and quantified by ... collected into PAXgene Blood
RNA Tubes (Qiagen, Valencia, CA; # 262115) and RNA
was prepared according to the manufacturer's protocol.
Spleens were removed and frozen in liquid n...
... 259:L262-269.
32. Keeton MR, Curriden SA, van Zonneveld AJ, Loskutoff DJ: Identifi-
cation of regulatory sequences in the type 1 plasminogen
activator inhibitor gene responsive to transforming growth
factor beta. ... Kaetzel DM: Auto-
crine platelet-derived growth factor-dependent gene
expression in glioblastoma cells is mediated largely by acti-
vation of the transcription factor sterol...
... have been attributed to its immunostimulatory,
anti-oxidant and anti-inflammatory activities. In this
study, we used human promonocytic U937 cells to inves-
tigate the modulatory effects of ginseng ... ginseng or ginseng extract have been shown to
have anti-inflammatory effects such as suppressing the
expression of proinflammatory cytokines or mediators.
For instance, ginsan, a polysacc...
... subcutane-
ous tumors developed to more than 1,500 mg, mice were
euthanized and tumors were dissected and mechanically
dissociated into equal pieces to be transplanted into the
flank areas of a new ... facilitate apoptosis. In this case,
downregulation of c-Myc expression by ApoG2 on one
hand could let cells away from c-Myc-induced apoptosis
and on other hand led to cell cycle arr...