... which included the frequency and type of emergency. The frequency of aircraft diversion was also investigated. All data regarding names, ages and the sex of the patient and the name of the air- line ... in the data analysis, literature search, revision of the bibliography, the revision and editing of most of the manuscript. BM participated in the da...
Ngày tải lên: 25/10/2012, 10:31
... was used at a concentration of 10μM for the indicated times. The PKA activator, forskolin (Sigma) was used at a concentration of 5 μM for the indicated times. Immunofluorescenct confocal microscopy. ... ratio of 1:200, and the lysate and antibody were incubated with gentle rocking overnight at 4 ° C. Protein A/G agarose was then added for 6h, after which time...
Ngày tải lên: 03/11/2012, 11:17
Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
... basis for structure-activity studies. The high selectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen- sional structures and the relative ... domain of each subunit contri- butes to the formation of the hydrophilic pore. ACh binding protein has structural and functional homology to the extracellular lig...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: "Syntactic and Semantic Kernels for Short Text Pair Categorization" docx
... b; 0 otherwise. (3) where s 1 a and s 2 b indicate the concatenation of the sequences s and t with the argument nodes, a and b, respectively and σ(h(a),h(b)) is 1 if the children of a and b are ... P 2 and sum the obtained contri- bution, where P 1 and P 2 are the set of PASs of the first and second answer 3 . Although different ker- nels can be used for...
Ngày tải lên: 22/02/2014, 02:20
Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf
... knowledge of the structural and functional properties of IBs drawn researchers’ attention to the possibility to control the conformation of aggregated proteins, paving the way for the use of IBs ... increased synthesis rates of DnaK, GroEL chaperones and Lon (the main heat-shock pro- tease) have been found to last for the whole length of overproduction. The...
Ngày tải lên: 06/03/2014, 00:20
Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... absence of tunicamycin. Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosy- lated form of the ... mutant forms of HFE and incubated the cells in the presence and absence of tunicamycin. Drug treatment resulted in the accumulation of an unglycosylated...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitro- gen of Asn114¢ of the other subunit. The contacts of the ribityl chain to His89 and Lys138¢ ... decades, the chemical nature of the second LS substrate, the four-carbon precursor of the pyrazine ring, remained unknown for a long time. The...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf
... excluded. Application of procheck [76] revealed that the stereochemical quality of the model was reliable, with 84% of the residues in the most favored regions of the tetramer model and none in disallowed ... strands b2 and b6 of neighboring monomers. The p26 modifications examined in this study are not located in either of these strands and they are not con- side...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
... terminal end and C2 defines the domain closest to the cell membrane. A comparison of the inferred amino acid sequence of the C1 and C2 domains reveals that only the approximate spacing of the 10 cysteine ... Lophochotrozoans, the third major group of bilaterian animals, have remained in the background of the molecular survey of metazoan development. We report...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
... Introduction of the point mutation, and absence of unintended mutations were confirmed by DNA sequencing. The expression and purification of the mutant enzyme was performed by the same procedure as that of ... is the initial velocity of the reaction, V max is the maximum velocity, [S] is the concentration of citrate, K s is the dissociation constant of the...
Ngày tải lên: 08/03/2014, 22:20