... Introduction of the point mutation, and absence of unintended mutations were confirmed by DNAsequencing. The expression and purification of the mutantenzyme was performed by the same procedure as that of ... is the initial velocity of the reaction, Vmaxis the maximum velocity, [S] is the concentration of citrate, Ksis the dissociation constant of the enzyme-citrate (ES) com-plex, and a, b, and ... phosphorylationbetween the AclAB complex (holoenzyme) and the AclAsubunit alone (Fig. 6). We then followed the phosphoryla-tion levels of the AclAB complex and AclA subunit atvarious time intervals. The phosphorylation...