... of these signaling events have yet to beinvestigated, many of the molecular interactions down-stream of the Eph and ephrin molecules appear to lead to the regulation of the cytoskeleton of the ... markers. The molecular sizes of each band are97.0, 66.0, 45.0, 30.0, 20.1 and 14.4 kDa from the top to the bottom. Lane 1: the three-component complex of GST-PDZ, ephrinB2(301–333)-pY304 and the ... the binding affinityof ephrinB2(301–333) to the SH2 domain of Grb4 and to the PDZdomain of PDZ-RGS3. In the combination experiments, the two Cterminal tyrosine residues were ignored due to their...