Plant cell culture
The Use of Plant Cell Biotechnology for the Production of Phytochemicals
... establishment of cell suspension cultures; (5) analysis of metabolite levels in cell suspension cultures; (6) selection of cell lines based on single cells; (7) analysis of culture stability; ... on the cells is, however, possible. Therefore, it is not possible to use a universal screening platform for plant cell cultures. Instead, a specific screening for a particular plant cel...
Ngày tải lên: 25/10/2013, 05:20
Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... in a mamma- lian cell culture system. Insect Biochem Mol Biol 33, 1239–1247. 22 Dinan L, Savchenko T & Whiting P (2001) On the dis- tribution of phytoecdysteroids in plants. Cell Mol Life Sci ... that the CHO cell culture assay system can be validly employed to characterize individual insect EcR–USP heterodimers for their responsiveness to agonists and potentiators. Utility of t...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... cells were obtained per 500 mL culture of 8-day-old +P i and –P i cells, respectively). Eight days following subculture of the tomato cells into –P i culture media, secreted (culture media) AP activity ... –P i cell culture being resupplied with 2.5 m M P i . All subsequent studies were performed using cell culture media filtrate from 8-day-old –P i cells. AP activity staining of...
Ngày tải lên: 23/03/2014, 21:20
basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
... of cells cultured and the risk that these cells might contain, and transmit, infectious agents. For example, culture of primate cells, transformed human cell lines, mycoplasma-contaminated cell ... the control DNA as internal standard (b). Cell cultures A, C, and E are mycoplasma positive; cell culture B is mycoplasma negative. The analy- sis of cell culture D is not evaluable...
Ngày tải lên: 08/04/2014, 12:50
plant cell biology - william v. dashek
... integration of cellular function in complex tissues. Page i Plant Cell Biology Structure and Function Brian E. S. Gunning Plant Cell Biology Group and Cooperative Research Centre for Plant Science ... larger companions. Plant tissues are composed of the non-living extracellular region and the living protoplasm of the cells proper. The former consists of intercellular space...
Ngày tải lên: 08/04/2014, 12:52
cancer cell culture, methods and protocols
... Langdon Basic Techniques of Cell Culture 21 2.4. Cloning 1. Cells. 2. Cell culture medium. 3. Trypsin-EDTA. 4. Hemocytometer or cell counter. 5. Petri dishes. 6. Agar. 2.5. Cell Counting 2.5.1. Hemocytometer ... increasing percentage of cells will undergo cell division. The initial cell density is a factor here and the higher the cell density, the more rapidly the culture...
Ngày tải lên: 10/04/2014, 11:11
cell culture
... replicate cultures are possible. The cells are grown in Rose chambers under a cellophane membrane. In many in- stances the monolayer cultures are initiated from explants. The cells in this culture ... for growing mass cultures is clearly related to the particular cell strain or cell line to be used. If diploid, anchorage- dependent cells are employed, then monolayer cell culture...
Ngày tải lên: 10/04/2014, 11:12
epithelial cell culture protocols
... types of cells, including smooth muscle cells, mesenchymal cells, vessel forming cells, nerve cells, blood cells, including immune cells and gastric gland cells. Gastric epithelial cells can ... procedures of HLE cell culture, so that scientists who are unfamiliar with this culture may carry it out successfully. The sources of HLE cells, explant culture, harvest, subculture, storage, .....
Ngày tải lên: 11/04/2014, 01:27