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analysis and determination of the stress intensity factor of load-carrying cruciform fillet welded joints

Tài liệu TREES OF THE NORTHERN UNITED STATES THEIR STUDY, DESCRIPTION AND DETERMINATION FOR THE USE OF SCHOOLS AND PRIVATE STUDENTS doc

Tài liệu TREES OF THE NORTHERN UNITED STATES THEIR STUDY, DESCRIPTION AND DETERMINATION FOR THE USE OF SCHOOLS AND PRIVATE STUDENTS doc

... pollen.[Pg 25] The last of the four kinds of parts is found in the center of the flower, and is called the pistil. It is this part which forms the fruit and incloses the seed. The stamens and the pistil ... other time of the year. The plan of branching, the position, number, size, form, color, and surface of buds, as well as the arrangement of the leaves within the bud and the peculiarities of ... twigs of the Ailanthus, or the drooping twigs of the Weeping Willow with the erect growth of the Lombardy Poplar, give contrasts of the strongest character. In the same way, the directions the...
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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc

Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc

... all the SMAD proteins of the HTFN and APC and b-catenin-related factors.Groups C and D involve smaller functional sets.Group C contains the Rel family and CRE bindingfactors involved in the ... functions, and topologi-cal features retain functionality and phylogeny. How-ever, the nature of the connections between thesefactors needs to be understood at the level of the pro-tein domain. The ... [47] and other related factors such as rox [48], mxi [47], miz-1[49] TRRAP, GNC5, bin-1 [50]. Group F contains90% of the members of the nuclear receptor hormonesuperfamily of the HTFN (they...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... (Met1)-ONC(M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis wasconfirmed by the disappearance of the (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spectrawere used to calculate the fraction of Pyr-ONC (M23L), ... 818.87) and Pyr-ONC (M23L) (theoretical molecular mass11 801.60), and expressing it as the fraction of hydrolyzed(Met1)-ONC (M23L).Figure 2 shows the results of the characterization of the hydrolysis...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, ... affect the binding of ATP to GlnK [12]. Therefore, we investigated the binding of TnrA to GlnK in the presence of differentmixtures of Mg2+or Mn2+with the effector moleculesATP and 2-oxoglutarate. ... until the late exponential growth phase, and the cells were then washed and resuspended inSMM without nitrate, and finally incubated for afurther 20 min. Samples were taken before and afterthe...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

... region of the 1H,15N-HSQC spectrum of the C-terminal domain of human eRF1 illustrating the presence of two conformational states of the protein.Fig. S2. Plot of the number and distribution of the NOEs ... supported by the existence of strong cros-speaks between the signal of the HN of Gly337 in the open conformer and the signal of the water protons.All of these observations indicate that the loop ... sizes of the relative populations and possibly the rate of conformerinterconversion are sensitive to the environment of the domain.For the great majority of the residues in the minido-main, the...
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Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

... enzyme activesites and groups of the reactant transition state (Sà)[2] ;the strength of the n oncovalent interaction between enzymeactive sites and Sàdetermines the stability of the enzyme–transition ... state (ESà) complex and the rate of the reaction.Thus, in theory, modifications of the enzymatic specificitycould be accomplished by changing a ctive-site residues. The role of the noncovalent interactions ... solate the DGàcorresponding to the energy of the hydrogen bond between residue 39 and 3-OH (/3), which was added to t he energy of /4e and /4a interactions.Results and Discussion The induction of...
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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot

Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot

... [36,37]. Furthermore, the analysis of the calculated structures revealed a short distancesbetween NH of Leu7 and the side chain carboxyl group of the Asp6 residue (< 0.28 nm), and NH of Leu7 ... isomerism.Characterization of the secondary structure of these twoligands of ODN receptors should provide useful informationfor the design of potent, stable and selective agonists and antagonists of these receptors. ... residue and C¼OofLeu5(< 0.24 nm). The proximity of thesetwo C¼O acceptors for one NH donor could explain the slowexchange H/D observed for NH of the Leu7 residue. Analysis of NMR data of cyclo128[D-Leu5]OP,...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc

Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc

... phosphoryla-tion is one of the major mechanisms used to control the function, localization and stability of proteinsinside cells [1]. Therefore, the analysis of protein kinaseactivity and the phosphorylation ... when incubated with eitherCK2a or CK2 holoenzyme in the presence of ATP and Mg2+(lanes 6 and 9). The absence of either CK2(lane 3), Mg2+(lanes 4 and 7) or ATP (lanes 5 and 8)completely abolished ... in the presence or absence of ATP and ⁄ or Mg2+, and the mixtures were analyzed by phospho-affinity gel electro-phoresis. CBB staining of the phospho-affinity gelshowed that the mobility of...
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Báo cáo khoa học: The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene pot

Báo cáo khoa học: The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene pot

... Control of the level of presenilins and its coordination with othercomponents of the c-secretase complex are likely to betightly regulated and we studied the transcriptionalcontrol of the PS1 ... activity,indicating the crucial importance of these two Etsmotifs [15]. In addition to controlling the level of geneexpression, Ets factors may direct the choice of the promoter elements in play, and therefore, ... 1821A,1810A, 1801A and 1790A. The common sense primer was1676ST and included and EcoRI site. The same series of inserts was inserted into the EcoRI and XhoI sites of pACT2 in order to also test the CHD3...
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Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx

Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx

... report the effects of these mutations on the thermodynamic properties of the FAD and also theirconsequences on the kinetics of FAD reduction. The thermodynamic properties of the wild-type and mutant ... flavin cofactors, and also of the haems in the case of NOS and P450 BM3. Inspectroelectrochemical titrations of the isolated domains, the lack of overlapping spectral contributions from othercofactors ... bleaching of the absorption in the region of the two major absorption bands of the flavin, while there is development, and then decay, of absorption at longer wave-length due to the formation of the...
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