viral vectors for gene therapy
viral vectors for gene therapy
... Virus Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G. Hawley 467 26 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard ... vol. 76: Viral Vectors for Gene Therapy: Methods and Protocols Edited by: C. A. Machida © Humana Press Inc., Totowa, NJ 1 Use of the Herpes Simplex Viral Genome to Construc...
Ngày tải lên: 11/04/2014, 10:39
nonviral vectors for gene therapy, methods and protocols
... acid side chains for a DNA carrier. Bioconjugate Chem. 9, 476–481. Cationic _ -Helical Peptides 11 11 From: Methods in Molecular Medicine, vol. 65: Nonviral Vectors for Gene Therapy Edited by: ... the Fig. 3. Formation of peptide–DNA complex and its transfer pathway into cell. M E T H O D S I N M O L E C U L A R M E D I C I N E TM Edited by Mark A. Findeis Nonviral Vectors for...
Ngày tải lên: 11/04/2014, 09:59
vectors in gene therapy
... expression. VIRAL VECTORS USED FOR GENE THERAPY Based on the virus life cycle, infectious virions are very efficient at transferring genetic information. Most gene therapy experiments have used viral vectors ... vectors for gene therapy, the relatively low levels of gene expression achieved in animals are prob- lematic. For currently selected genes used for gen...
Ngày tải lên: 10/04/2014, 22:11
![Báo cáo y học: "Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors"](https://media.store123doc.com/images/document/12/to/qp/medium_qpq1351678087.png)
Báo cáo y học: "Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors"
... INTRODUCTION Gene therapy provides a novel method to repair damaged bone by using bone morphogenetic protein (BMP). BMP gene vectors can be divided into DNA, viral, and cell vectors [10, 19]. ... adenoviral vectors, which induce the host immune response, consist of directly injected viral particles and viral genome–expressed proteins that include viral proteins and fore...
Ngày tải lên: 31/10/2012, 17:08
Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... the lentiviral vector may be used for the delivery and expression of the IDUA gene to cells in vivo for treatment of MPS I. Keywords: MPS I; Hurler syndrome; a- L -iduronidase; gene therapy. Mucopolysaccharidosis ... followed by 30 cycles at 94 °Cfor1min,57°C (for the 5¢Alu-PCR) or 49 °C(forthe3¢Alu-PCR) for 1min,and72°C for 1 min. After this first amplification, a nested PCR wa...
Ngày tải lên: 24/03/2014, 04:21
gene therapy for hematological disorders
... modified aden- oviral vectors have been developed with minimal residual adenoviral genes. Non- human marker genes such as the Neo gene or suicide genes such as tk gene included in vectors for selection ... revolutionize the gene therapy field. Immune Responses to Vectors and Transgenes Immune responses against vector proteins or transgene-encoded proteins are clearly an obstacle...
Ngày tải lên: 10/04/2014, 22:10
gene therapy for liver disease
... the therapeutic gene. Alternatively, dif- ferent viral vectors as well as liposomes can be used for ex vivo gene transfer. 154 GENE THERAPY FOR LIVER DISEASE For in vivo gene therapy, the therapeutic ... LIVER-DIRECTED GENE THERAPY 169 estingly, the animals continued to express the transferred B-UGT gene and did not experience an increase in unconjugated serum biliru...
Ngày tải lên: 10/04/2014, 22:11
gene therapy for hiv infection
... retroviral-mediated gene transfer is currently the optimal gene transfer system available for use in HIV-1 gene therapy. The PRACTICAL ASPECTS OF GENE THERAPY FOR HIV 281 CHAPTER 11 Gene Therapy ... evaluated for their effectiveness for gene delivery. The viral- derived gene transfer systems have been extensively tested on both HSCs and PBL. Only the retroviral...
Ngày tải lên: 10/04/2014, 22:11
Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... Chromosome engineering: prospects for gene therapy. Gene Ther. 2002; 9(11):713-718. 85. Lipps HJ, Jenke ACW, Nehlsen K, et al. Chromosome-based vectors for gene therapy. Gene Ther. 2003; 304:23-33. ... Ikawa M, Tanaka N, Kao WWY, Verma IM. Generation of transgenic mice using lentiviral vectors: A novel preclinical assessment of lentiviral vectors for gene therapy....
Ngày tải lên: 03/11/2012, 10:01
sermina lieu phap gen human gene therapy
... dục. nguyenthanhloi_2@yahoo.com 54 TRƯỜNG ĐH TÂY NGUYÊN KHOA KHTN & CN LIỆU PHÁP GEN (Human Gene Therapy) GVHD: Võ Thị Phương Khanh SVTH: Nguyễn Thành Lợi Hồ Thị Lan Anh Nguyễn Thị Trang nguyenthanhloi_2@yahoo.com ... bắn gen, các vi đạn có bọc gen liệu pháp để đưa vào tế bào đích Kỹ thuật dùng súng bắn gen (gene gun): nguyenthanhloi_2@yahoo.com 14 Kỹ thuật liposom (liposom te...
Ngày tải lên: 06/09/2013, 22:49