small gtpases and their regulators, part f
small gtpases and their regulators, part f
... link signaling from one family member to another, as well as the use of shared regulators and effectors by different family members. Concurrent with the expanded complexity of Ras family biology, ... of chapters. In particular, we hope these volumes will provide valuable references and sources of information that will facilitate the efforts of newly incoming researchers to the study...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part a
... of buffer B, [2] PURIFICATION OF BACULOVIRUS-EXPRESSED Ras AND Rap, 19 30 vol of buffer B containing 0.5% (w/v) sodium cholate, 30 vol of buffer B containing 500 mM NaCI, and 30 vol of buffer ... buffer B, 30 vol of buffer B containing 0.5% Nonidet P-40 (NP-40), 30 vol of buffer B containing 500 mM NaCI, and 30 vol of buffer B. Unprocessed K-Ras(4B) is eluted in ten 1-ml fractions...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part b
... buffer F. After the column is washed with 15 ml of the same buffer, elution is performed with a 30-ml linear gradient of NaC1 (0-500 mM) in buffer F at a flow rate of 0.5 ml/min. Fractions of ... mg of protein) is diluted fivefold with buffer B and applied to a CM-Sepharose column (1.5 x 20 cm) equilibrated with buffer B. Elution is performed with 50 ml of buffer B followed by buffe...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part c
... Q-Sepharose Fast Flow col- umn preequilibrated in lysis buffer and washed with 20 ml of lysis buffer. Proteins are eluted with a 2 x 50-ml gradient of 0-200 mM NaC1 in lysis buffer and 2-ml fractions ... posttranslationally modified and unmodified Rab5 from Spodoptera frugiperda (Sf9) insect cells overex- pressing the protein. Purified posttranslationally modified and unmodifie...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part d
... activation of JNK, transfect one plate of cells with pKH3-JNK (and empty vector to normalize plasmid levels). To test the effect of putative TC10 effectors on JNK activity, cotransfect the effector ... concentration of recombinant, cleaved effector protein in a small volume (40-200 /zl) of binding buffer and incubate at 4 ° for 1 hr. (Alternatively, GST-TC10 and a cleaved effecto...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part e
... higher affinity for the GDP than for the GTP form of Rab proteins. To quantitate this and other interactions more fully and to obtain information on the dynamics, fluorescence from different ... study their function. Because each family contains a variety of isoforms, the techniques described for a particular GTPase family member are likely to be useful for other members of the same...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part g
... link signaling from one family member to another, as well as the use of shared regulators and effectors by different family members. Concurrent with the expanded complexity of Ras family biology, ... of chapters. In particular, we hope these volumes will provide valuable references and sources of information that will facilitate the efforts of newly incoming researchers to the study...
Ngày tải lên: 11/04/2014, 10:32
SMALL-SCALE MINES, THEIR CUMULATIVE ENVIRONMENTAL IMPACTS AND DEVELOPING COUNTRIES BEST PRACTICE GUIDELINES FOR WATER MANAGEMENT
... process, between regulators, miners and communities by the Department of Water Affairs and Forestry (DWAF). These BPG’s address issues that range from water and salt balances to small- scale mine ... the mine site and the body of water of at least 100m √ Store oil, fuel & chemicals safely in designated area outside of the buffer zone √ Locate toilets outside of the buffer...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... late in life. Invest Ophthalmol Vis Sci 39, S523. 72 Bai F, Xi JH, Wawrousek EF, Fleming TP & Andley UP (2003) Hyperproliferation and p53 status of lens epithelial cells derived from aB-crystallin ... 275, H2243–H2249. Small heat shock proteins and disease Y. Sun and T. H. MacRae 2624 FEBS Journal 272 (2005) 2613–2627 ª 2005 FEBS 91 Vandroux D, Schaeffer C, Tissier C, Lalande A,...
Ngày tải lên: 19/02/2014, 18:20
Báo cáo khoa học: "Self-Organizing Markov Models and Their Application to Part-of-Speech Tagging" potx
... do 1 sort meaningful value set V for f ; if |V | > 1 then 2 measure the contribution of f to t; if f contributes the most then select f as the best feature b; end end end if there is b selected ... as a meaningful value. We adopted the χ 2 -test to determine the difference between the distributions of the target feature before and after re- ferring to the value v. The use of χ 2...
Ngày tải lên: 31/03/2014, 03:20