small gtpases and their regulators, part c
small gtpases and their regulators, part c
... exocytic and endocytic transport processes. 1 They are localized to diverse intracellular compartments and participate in vari- ous steps of vesicular traffic. 1 In yeast, two Rab GTPases, Sec4p ... exocytic and endocytic transport processes. 1 They are localized to diverse intracellular compartments and participate in vari- ous steps of vesicular traffic. 1 In yeast, two Rab GT...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part a
... Martin, D. Viskochil, G. Bollag, P. C. McCabe, W. J. Crosier, H. Haubruck, L. Conroy, R. Clark, P. O'Connell, R. M. Cawlhon, M. A. Innis, and F. McCormick, Cell (Cambridge. Mass. ) 63, 843 (199(I). ... J. E. Childs, and C. J. Marshall, Cell (Cambridge, Mass.) 57, 1167 (1989). 5 S. Clarke, Annu. Rev. Biochem. 61, 355 (1992). J. F. Hancock, H. Patcrsom and C. J. Marshall,...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part b
... baculovirus carrying the cDNA of Rac2 is from P. Polakis and F. McCormick (Onyx Pharmaceuti- cals, Richmond, CA). GTPTS-Rac2 and GDP-Rac2 are prepared as de- scribed. 9 Methods Purification ... sequence for the Saccharomyces cerevisiae cell division cycle protein, Cdc42 (designated Cdc42Sc), which had been shown to be essential for proper assembly of the bud site. 6 The human cD...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part d
... ClaI CGG ~3CT AGC t GG~3 CGG CCG C~ lqGAA TTCnATC GAT i pKFLAG BamHI EeoRI ClaI Sill tGGA TCC~tGAA TI" ;C~ AtAT CGA qtGG CCG CCA TGG CC~ PRKT-GFP BamHI EcoRI ClaI ~~GA GAA ~TC pKNzz BamHI ... EeoRI ClaI ~GCIG CGG CCG CbA ATT C~ A~ pGBT10 BamHI AatI1 EcoRI iGGA TCCIIGAC GTCI~ VP16-CP BamHI AatlI EcoRI IGGA TCCJIGAC GTCtIGGT ACC I FIG. 1. Multiple cloning sites of express...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part e
... affect GFP fluorescence. If GFP fluorescence is dim, cells can be costained with commercially available GFP antibodies (Clontech, Palo Alto, CA) followed by secondary FITC or Alexa 488-coupled ... biosafety hood and process the cells at a laboratory bench. 4. Carefully pick up coverslip with forceps and wick away excess fluid using a Kimwipe or tissue. 5. Place coverslip (cells fac...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part f
... BamHI EcoRI BamHI ATGGCTTCTAGCTATCCTTATGACGTCCCTGACTATGCCAGCCTCGGA GGATCCCCGGGAATTCCCGGGGATCC pZBRII I~ M A S S Y P Y D V P D Y A S L G Sail Ndel Kpnl Sacll BamHI Sscll GGACCTTCTAGGTCGACCCATATGGTTAACGGTACCCGCGGATCC ... PP~,"E ~Nhel (1125) Scal-'~[~ +'" ] (3235) Notl (2635) ATGGCTTCTAGCTATCCTTATGACGTCCCTGAC k M A S S Y P Y D V P D BamHI TATGCCAGCCTCGGA GGACCTTC...
Ngày tải lên: 11/04/2014, 10:32
small gtpases and their regulators, part g
... superoxide. 6 CYTOPLASMIC AND NUCLEAR SIGNALING ANALYSES [1] A. C. IP: ShcA ShcC Blot: ShcA ShcC I 2 3 4 5 6 B. Do IP: ShcA ShcC Blot: ShcA ShcC 1 2 3 4 5 6 IP: ShcA ShcC Blot: ShcC ShcA 1 ... a significant signal, suggesting that the ShcA antibody recognizes both ShcA and ShcC (Fig. 2D, lane 2). In contrast, if an ShcC im- lnunoprecipitate from A673 cells, which lack ShcC...
Ngày tải lên: 11/04/2014, 10:32
rna polymerase and associated factors, part c
... Vitamins and Coenzymes (Part G) Edited by Frank Chytil and Donald B. McCormick Volume 123. Vitamins and Coenzymes (Part H) Edited by Frank Chytil and Donald B. McCormick Volume 124. Hormone Action (Part ... Sidney Fleischer and Becca Fleischer Volume 126. Biomembranes (Part N: Transport in Bacteria, Mitochondria, and Chloroplasts: Protonmotive Force) Edited by Sidney Fleischer...
Ngày tải lên: 11/04/2014, 10:30
SMALL-SCALE MINES, THEIR CUMULATIVE ENVIRONMENTAL IMPACTS AND DEVELOPING COUNTRIES BEST PRACTICE GUIDELINES FOR WATER MANAGEMENT
... Furthermore, new small- scale mine operators face technical barriers to participate in mining, including lack of skills in dealing with aspects such as complex metallurgical processes, practical mining ... • Collaboration forum and co-ordination • Technical support and appropriate technology • Best practice guidelines • Improved stakeholder participation in decision-making Best...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... mouse aA-crystallin gene induces cata- ract and cytoplasmic inclusion bodies containing the small heat shock protein aB-crystallin. Proc Natl Acad Sci USA 94, 884–889. 70 Liu J-P, Schlosser R, ... [61]. This change causes lens central core nuclear opacities, as does the R11 6C mutation. However, in contrast to R11 6C aA-crystallin, the R4 9C variant localizes to the cell nucleus and...
Ngày tải lên: 19/02/2014, 18:20