recombinant dna part b
recombinant dna part b
... /~g of lambda DNA < /b> under standard reaction conditions. ' All enzymes and DNAs were from Bethesda Research Laborato- ries, Inc. d Linear form III pBR322 DNA < /b> was prepared by incubation of ... Nussbaum, L. Tobias, D. E. Garfin, H. W. Boyer, and H. M. Goodman, J. Mol. Biol. 99, 237 (1975). to B. Hinsch and M R. Kula, Nucleic Acids Res. 8, 623 (1980). 6 ENZYMES IN RE...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part a
... making staggered cuts may be used, but the DNA < /b> termini must then be made blunt by biochemical methods. This has been done by removal of single-stranded termini by incubation with single-strand-specific ... rabbit/3-globin in monkey kidney cells (Table II). An in situ nucleic acid hybridization method for SV40 recombinants has been described. 4a In other experiments involving int...
Ngày tải lên: 11/04/2014, 10:25
recombinant dna part c
... linker in 1059 BamHI sites D BamHI, XhoI 9-22 XbaI linker in 2004 BamHI sites C XbaI 7-20 hhsbaml b1 89att int (XbaI)ninL44 C XbaI 5-18 A[sHindlII3-sHindlII5]~bio 256int (XbaI) [int-clII]cI857 ... R SaSa FI Bet H R (Xbo) :Xn/):, pad 29 Ba S~Sa ,i X1059 (X1274) X1672 b, o 256 I Ba SaSa Ba HHH H (Xba) (Xba trp E Ba SaSa i i R~ H R H H~F~- Sa S~ ~2004 (X2053) E...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part d
... can be purchased from Boehringer, Pharmacia, New En- gland BioLabs, or Bethesda Research Laboratories, and should be used as recommended by the supplier. Media, Nutritional Supplements, and Buffers ... plasmids allow the separate biosynthesis of both DNA < /b> strands of a recombinant < /b> plasmid in an ss form and thus overcome the drawbacks of the filamentous phage vectors described...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part e
... difficulty in using it. DNA < /b> recovered by other methods may not be clean enough. Gel buffers containing borate should not be used; borate inhibits solubili- zation of agarose gel by NaI. As a result ... scribed above. The tailed linker fragment (450 bp) is recovered by the glass bead method as described above, except that a somewhat larger amount of glass bead suspension and a lon...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part f
... hr) with 1/zg of ~bX174 RF I DNA < /b> in the standard restriction endonuclease assay buffer in a volume of 0.05 ml. The supercoiled ~bX174 DNA < /b> has been substituted for Adeno-2 DNA < /b> because neither ... Scienze Biochimiche, H Fa- coltd di Medicina, 80131 Napoli, Italy BERNHARD DOBBERSTEIN (26), European Molecular Biology Laboratory, D-6900 Heidelberg, Federal Republic of...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part g
... linear DNA < /b> molecule that is smaller in size by about 40%. For example, the form I DNA < /b> of the 2865-base pairs (bp) plasmid pGEM-1 exhibits the mobility of a linear DNA < /b> of approximately 1720 bp in ... clear microbead pellet from the buffer, hold the Eppendorf tube up to a light to make the pellet/buffer boundary visible by the difference in diffraction. The buffer remai...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part h
... Strasbourg, France [3] HETEROLOGOUS PROTEIN PRODUCTION BY B. brevis 27 Preparation of Plasmid DNA < /b> from Bacillus brevis Cells The method of Birnboim and Doly 12 can be used to obtain plasmid ... Bacteriol. 170, 935 (1988). [3] HETEROLOGOUS PROTEIN PRODUCTION BY B. brevis 25 Preservation of Bacteria Bacillus brevis cells, including those having plasmids, can be preserved at...
Ngày tải lên: 11/04/2014, 10:26
recombinant dna part i
... streptavidin has been attached. 13 The strand labeled through the incorporated PCR primer will be bound to the solid support, and the unbound strand can be removed. The bound ssDNA is subsequently ... regular agarose gel. The ssDNA is visible after the bromphenol blue has migrated about 2 cm as a discrete fraction migrating ahead of the dsDNA. If a ssDNA fraction is visible by ethidium...
Ngày tải lên: 11/04/2014, 10:27
UNIT 5: ILLITERACY- PART B: SPEAKING
... mountainous areas. - Students cannot buy all the required books. What are your suggested solutions to these problems? To solve these problems I think we should: • Build more schools. • Provide ... transportation, books, money … • Send teachers to remote areas. TASK 1: WORK IN PAIRS. Match each problem in A with its appropriate solutions in B A B 1. Many students can’t buy a...
Ngày tải lên: 18/09/2013, 03:10