protein purification protocols, 2nd ed

... industries. Indeed, seed proteins were among the earliest of all proteins to be studied in detail, with wheat gluten being isolated in 1745 (1), the Brazil nut glob- ulin edestin crystallized in 1859 ... spe- cialized procedure for the extraction of seed proteins from cereals, based on the classical Osborne fractionation. In addition, two rapid methods are described for the extraction of leaf...

... Molecular Biology TM Edited by Bryan John Smith Protein Sequencing Protocols VOLUME 211 SECOND EDITION Edited by Bryan John Smith Protein Sequencing Protocols SECOND EDITION SDS-PAGE for Protein Sequencing ... polyethylenimine may reduce this loss, as indicated by an observed improved retrieval of radiolabeled bradykinin from polypropylene tubes (increased from 30% to 65% yield). Tubes...

... supernatant can be used directly for enzyme activ- ity measurements (see Note 4). 3.2. Extraction of Cereal Seed Proteins Using a Modified Osborne Procedure The procedure is based on Shewry et ... of all proteins to be studied in detail, with wheat gluten having been isolated in 1745 (I), the Brazil nut globulm, edestm, crystalhzed m 1859 (2) and a range of globulin storage proteins .....

... Molecular Biology, vol. 252: Ribozymes and siRNA Protocols, Second Edition Edited by: M. Sioud © Humana Press Inc., Totowa, NJ 1 Ribozyme- and siRNA-Mediated mRNA Degradation A General Introduction Mouldy ... specified in the kit. The NTPs are used at high concentrations, about 5–7 mM each. Although this will compromise the transcript yields, reduce GTP concentration to 2 mM, thus reducing...

... Toxins: Methods and Protocols, edited by Otto Holst, 2000 144. Calpain Methods and Protocols, edited by John S. Elce, 2000 143. Protein Structure Prediction: Methods and Protocols, edited by David Webster, ... Methods and Protocols, edited by B. Paul Morgan, 2000 149. The ELISA Guidebook, edited by John R. Crowther, 2000 148. DNA Protein Interactions: Principles and Protocols (2nd...