protein lipidation protocols

... and monoclonal antibody against the GPI-anchored marker protein (the concentration should be determined experimentally). Protein Lipidation Protocols Edited by Michael H. Gelb Methods in Molecular ... cells. J. Biol. Chem. 267, 24,611–24,619. GPI-Anchored Proteins 23 23 From: Methods in Molecular Biology, Vol. 116: Protein Lipidation Protocols Edited by: M. H. Gelb © Humana Pre...

Ngày tải lên: 11/04/2014, 10:12

... of a soluble protein for membranes, which can thereby affect the protein s localization and function. This is the case for both solely palmitoylated proteins and dually lipidated proteins. This ... indicator of proper folding. Protein Acyltransferase Protein Acylthioesterase Fig. 2. Kinetic trapping model of dually lipi- dated proteins [11]. The farnesylated protein (gray rectangle)...

Ngày tải lên: 07/03/2014, 05:20

... Biology TM Edited by Bryan John Smith Protein Sequencing Protocols VOLUME 211 SECOND EDITION Edited by Bryan John Smith Protein Sequencing Protocols SECOND EDITION SDS-PAGE for Protein Sequencing 19 2 SDS ... rapid, and is suitable for proteins of either acidic or basic pI. The last is because the protein is reacted with SDS, which binds to the protein in the approximate ratio 1...

Ngày tải lên: 11/04/2014, 07:13

... Biology TM HUMANA PRESS Protein Kinase Protocols Edited by Alastair D. Reith HUMANA PRESS Methods in Molecular Biology TM VOLUME 124 Protein Kinase Protocols Edited by Alastair D. Reith Protein Kinase-Mediated ... supressor of ras is ceramide-activated protein kinase. Cell 89, 63–72. 30. Xing, H., Kornfeld, K., and Muslin, A. J. (1997) The protein kinase KSR interacts with 14-3-...

Ngày tải lên: 11/04/2014, 10:12

... Collect fractions, 10 mL, and assay for the presence of protein and protein phosphatase actwity (see Subheading 3.2.) A single peak of protein phosphatase activity that elutes near the midpoint ... Introduction 1.1. Prokaryotic Protein- Serine/Threonine Phosphatases: A Brief Review 1.1.1. Why Study Protein Phosphorylation Events in Prokaryotes? As this chapter deals with the p...

Ngày tải lên: 11/04/2014, 10:12

... These proteins can only be solubilized following chemical hydrolysis or proteolytic cleavage. Membrane-bound proteins can be subdivided into integral membrane proteins, where the protein or proteins ... Molecular Biology TM Methods in Molecular Biology TM Protein Purification Protocols Second Edition Edited by Paul Cutler VOLUME 244 Protein Purification Protocols Second Edition Edit...

Ngày tải lên: 11/04/2014, 10:12

... of glycoproteins and in removal of glycoprotein contaminants from other proteins, and is also capable of high specificity. Affinity methods that rely on interactions of the target protein ... studies of seed storage proteins, whereas the terms albumin and globulin have become accepted into the general vocabulary of protein chemists. Three detailed protein extraction protocols ar...

Ngày tải lên: 11/04/2014, 10:12

... detection Lmearrty” by Stained densitometry Stained protein protein ng protein/ mm2 approx by rwse, ng decolorizatton color eye densrtometry protein/ mm2 Ponceau S 0.1% w/v Water; few min Water, ... of proteins by ton-exchange chromatography Proviso 1s that the protein should bear charge, t e , the solutton pH should not be equal to the protems p1. With that condttton satisf...

Ngày tải lên: 11/04/2014, 10:13

... portions (5-20 pL) of one protein ( protein Q”) from the ITC injection syringe into the calorim- eter cell (approx 1.5 mL) containing the second protein component ( protein P”). Concentrations ... protein will be affected by ligand binding or complex formation, and the energetics of this can be followed by DSC experiments and may be used, at least in principle, to explore protei...

Ngày tải lên: 11/04/2014, 10:13

... Biology RNA Protein Interaction Protocols Edited by Susan R. Haynes VOLUME 118 HUMANA PRESS HUMANA PRESS Methods in Molecular Biology Edited by Susan R. Haynes RNA Protein Interaction Protocols Joining ... 364–368. RNA Protein Crosslinking 21 21 From: Methods in Molecular Biology, Vol. 118: RNA -Protein Interaction Protocols Edited by: S. Haynes © Humana Press Inc., Totowa, NJ 3 RNA...

Ngày tải lên: 11/04/2014, 10:31