pcr cloning protocols
pcr cloning protocols - harry w. janes, bing-yuan chen
... Beijing, China xiv Contributors PCR: Basic Principles 1 I PERFORMING AND OPTIMIZING PCR PCR: Basic Principles 3 3 From: Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd Edition Edited ... 2002 193. RT -PCR Protocols, edited by Joseph O’Connell, 2002 192. PCR Cloning Protocols, 2nd ed., edited by Bing-Yuan Chen and Harry W. Janes, 2002 191. Telomeres and Telome...
Ngày tải lên: 08/04/2014, 12:52
pcr cloning protocols
... and Higuchi, R. (1989) Avoiding false positives with PCR Nature 339, 237,238. 8. Orrego, C (1990) Organizing a laboratory for PCR work. PCR Protocols. A Guide to Methods and Appltcations (Innis, ... phage 1 genome (see Notes 6,7) 2. AmpliWax PCR Gems (Perkm-Elmer). 3. Perkin-Elmer GeneAmp PCR Instrument System and PCR tubes with which the XL PCR reagents have been optimi...
Ngày tải lên: 11/04/2014, 10:01
pcr sequencing protocols
... the PCR amplifications (1-4). Therefore, a number of protocols have been devised that address these technical issues, and allow effi- cient sequencing of either conventional double-stranded PCR ... 1-4). Many of these protocols are described in detail in this volume. An unrelated, but equally frustrating obstacle to the sequencing of PCR products arises when multiple PCR produc...
Ngày tải lên: 11/04/2014, 10:02
Cloning genes by PCR
... secondary PCR amplification; lane 1 represents amplification from primary PCR 1 and lane 2 represents amplification from primary PCR 3. consuming steps. Multiplex restriction site PCR (mrPCR) eliminates ... amplification repeated. An example of the result from an IPCR experiment is shown in Figure 10.7. 10.5 Multiplex restriction site PCR (mrPCR) Although IPCR is a relatively rapid way...
Ngày tải lên: 25/10/2013, 22:20
Purification and cloning of PCR products
... for cloning PCR products by exploiting the additional A added by Taq DNA polymerase. 116 PCR 6.4 Approaches to cloning PCR products Essentially any cloning vector can be used for cloning a PCR ... protecting Purification and cloning of PCR products 121 PCR products with terminal half restriction sites added by PCR primers Blunt-end polish then ligate PCR products Rest...
Ngày tải lên: 25/10/2013, 22:20
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization doc
... of 100 lL. PCR was performed in a Thermal Cycler (PerkinElmer 2400) for nine cycles: 30 s at 94 °C, 30 s at 50 °Cand45sat68°C. The PCR products were then puri®ed using H igh Pureä PCR Product ... proA12 lacY1 galK2 rspL20 mtl-1xyl-5) [20] was used for cloning of the mutated XIs using pGEMÒ-T Easy vector (Promega, Madison, WI, USA). PCR mutagenesis Random mutagenesis of the XI gene (...
Ngày tải lên: 31/03/2014, 15:20
pcr protocols 2d ed - john m. s. bartlett
... in a PCR laboratory. 16 McDonagh Methods in Molecular Biology TM Methods in Molecular Biology TM PCR Protocols S ECOND E DITION Edited by John M. S. Bartlett David Stirling Volume 226 PCR Protocols S ECOND ... (Roche). Subcycling PCR 101 7 From: Methods in Molecular Biology, Vol. 226: PCR Protocols, Second Edition Edited by: J. M. S. Bartlett and D. Stirling © Humana Press In...
Ngày tải lên: 08/04/2014, 12:52
rt-pcr protocols - joe o’connell
... O’Connell RT -PCR Protocols VOLUME 193 Edited by Joe O’Connell RT -PCR Protocols RT -PCR in Biomedicine 5 Many quantitative RT -PCR approaches are based on competitive PCR (6). Essentially, a control PCR ... Transcripts by Anti-Sense RT -PCR Michael C. Yeung and Allan S. Lau 341 PART X. RT -PCR IN CDNA CLONING 26 RT -PCR in cDNA Library Construction Vincent Healy 349 27 An RT...
Ngày tải lên: 08/04/2014, 12:52
pcr mutation detection protocols
... Polyacrylamide Gel Electrophoresis 1 1 From: Methods in Molecular Biology, vol. 187: PCR Mutation Detection Protocols Edited by: B. D. M. Theophilus and R. Rapley © Humana Press Inc., Totowa, ... Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbour, NY. 150 Wells Internal Labeling of DNA Probes 13 13 From: Methods in Molecular Biology, vol....
Ngày tải lên: 11/04/2014, 07:13
pcr protocols
... This cDNA may be used directly in PCR reactions or may be modified further (see Note 18). 3.2. Performing PCR 3.2.1. Basic PCR Protocol The ideal way to perform PCR is in a dedicated room, using ... to carry out PCR, the isolation of DNA and RNA as templates, the basic PCR protocol, and several common methods for analyzing PCR products. 2. Materials 2.1. Preparation for...
Ngày tải lên: 11/04/2014, 10:01