pcr cloning protocols
pcr cloning protocols - harry w. janes, bing-yuan chen
... Beijing, China
xiv Contributors
PCR: Basic Principles 1
I
PERFORMING AND OPTIMIZING PCR
PCR: Basic Principles 3
3
From:
Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd Edition
Edited ... 2002
193. RT -PCR Protocols, edited by Joseph O’Connell, 2002
192. PCR Cloning Protocols, 2nd ed., edited by Bing-Yuan Chen
and Harry W. Janes, 2002
191. Telomeres and Telome...
pcr cloning protocols
... and Higuchi, R. (1989) Avoiding false positives with PCR Nature 339,
237,238.
8. Orrego, C (1990) Organizing a laboratory for PCR work. PCR Protocols. A Guide
to Methods and Appltcations (Innis, ... phage 1 genome
(see Notes 6,7)
2. AmpliWax PCR Gems (Perkm-Elmer).
3. Perkin-Elmer GeneAmp PCR Instrument System and PCR tubes with which the
XL PCR reagents have been optimi...
pcr sequencing protocols
... the PCR amplifications (1-4). Therefore, a number of
protocols have been devised that address these technical issues, and allow effi-
cient sequencing of either conventional double-stranded PCR ... 1-4). Many of these
protocols are described in detail in this volume.
An unrelated, but equally frustrating obstacle to the sequencing of PCR products
arises when multiple PCR produc...
Cloning genes by PCR
... secondary PCR
amplification; lane 1 represents amplification from primary PCR 1 and lane 2
represents amplification from primary PCR 3.
consuming steps. Multiplex restriction site PCR (mrPCR) eliminates ... amplification repeated.
An example of the result from an IPCR experiment is shown in Figure 10.7.
10.5 Multiplex restriction site PCR (mrPCR)
Although IPCR is a relatively rapid way...
Purification and cloning of PCR products
... for cloning PCR products by exploiting the additional A
added by Taq DNA polymerase.
116 PCR
6.4 Approaches to cloning PCR products
Essentially any cloning vector can be used for cloning a PCR ... protecting
Purification and cloning of PCR products 121
PCR products with terminal
half restriction sites added
by PCR primers
Blunt-end polish then
ligate PCR products
Rest...
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization doc
... of 100 lL. PCR was performed in a Thermal Cycler
(PerkinElmer 2400) for nine cycles: 30 s at 94 °C, 30 s at
50 °Cand45sat68°C. The PCR products were then
puri®ed using H igh Pureä PCR Product ... proA12
lacY1 galK2 rspL20 mtl-1xyl-5) [20] was used for cloning
of the mutated XIs using pGEMÒ-T Easy vector (Promega,
Madison, WI, USA).
PCR mutagenesis
Random mutagenesis of the XI gene (...
pcr protocols 2d ed - john m. s. bartlett
... in a PCR laboratory.
16 McDonagh
Methods in Molecular Biology
TM
Methods in Molecular Biology
TM
PCR
Protocols
S
ECOND
E
DITION
Edited by
John M. S. Bartlett
David Stirling
Volume 226
PCR
Protocols
S
ECOND
... (Roche).
Subcycling PCR 101
7
From: Methods in Molecular Biology, Vol. 226: PCR Protocols, Second Edition
Edited by: J. M. S. Bartlett and D. Stirling © Humana Press In...
rt-pcr protocols - joe o’connell
... O’Connell
RT -PCR
Protocols
VOLUME 193
Edited by
Joe O’Connell
RT -PCR
Protocols
RT -PCR in Biomedicine 5
Many quantitative RT -PCR approaches are based on competitive PCR (6).
Essentially, a control PCR ... Transcripts by Anti-Sense RT -PCR
Michael C. Yeung and Allan S. Lau 341
PART X. RT -PCR IN CDNA CLONING
26 RT -PCR in cDNA Library Construction
Vincent Healy 349
27 An RT...
pcr mutation detection protocols
... Polyacrylamide Gel Electrophoresis 1
1
From:
Methods in Molecular Biology, vol. 187: PCR Mutation Detection Protocols
Edited by: B. D. M. Theophilus and R. Rapley © Humana Press Inc., Totowa, ... Molecular Cloning: A Laboratory
Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbour, NY.
150 Wells
Internal Labeling of DNA Probes 13
13
From:
Methods in Molecular Biology, vol....
pcr protocols
... This cDNA may be used directly in PCR reactions or may be modified
further (see Note 18).
3.2. Performing PCR
3.2.1. Basic PCR Protocol
The ideal way to perform PCR is in a dedicated room, using ... to carry out PCR, the isolation of
DNA and RNA as templates, the basic PCR protocol, and several
common methods for analyzing PCR products.
2. Materials
2.1. Preparation for...