... Findeis
Nonviral
Vectors for
Gene Therapy
Methods and Protocols
Humana Press
Humana Press
Edited by
Mark A. Findeis
Nonviral
Vectors for
Gene Therapy
Methods and Protocols
20 Niidome and Aoyagi
internalization of ... 3. Formation of peptide–DNA complex and its transfer pathway into cell.
M E T H O D S I N M O L E C U L A R M E D I C I N E
TM
Edited by
Mark A. F...
... Krauss
Recombinant
Antibodies for
Cancer Therapy
Methods and Protocols
Methods and Protocols
Generation of Antibody Molecules 21
49. Better, M., Chang, C. P., Robinson, R. R., and Horwitz, A. H. (1988) ... Overall response
Generation of Antibody Molecules 3
3
From:
Methods in Molecular Biology, vol. 207: Recombinant Antibodies for Cancer Therapy: Methods and Protocols...
... Robbins, P. D. and Ghivizzani, S. C. (1998) Viral vectors for gene therapy. Pharm. Ther.
80, 35–47.
12. Zhang, W. W. (1999) Development and application of adenoviral vectors for gene therapy
of ... both suicide genes and
immune enhancing genes. The transgenes containing both a suicide gene and granulo-
cyte macrophage colony stimulating factor (GM-CSF) or interleukin (IL...
... Virus Type 1-Based Vectors
for Gene Delivery to Human Hematopoietic Stem Cells
Ali Ramezani and Robert G. Hawley 467
26 Semliki Forest Viral Vectors for Gene Transfer
Jarmo Wahlfors and Richard A. ... 493
Contents
x
27 Semliki Forest Virus (SFV) Vectors in Neurobiology
and Gene Therapy
Kenneth Lundstrom and Markus U. Ehrengruber 503
28 Semliki Forest Virus Vectors f...
... predetermined.
Methods and Protocols
Methods and Protocols
Gene
Therapy
of Cancer
M E T H O D S I N M O L E C U L A R M E D I C I N E
TM
Humana Press
Edited by
Wolfgang Walther
Ulrike Stein
Gene
Therapy
of ... Breast Cancer
Hardev S. Pandha and Nicholas R. Lemoine 523
C:
Anti-Oncogene and Suppressor Gene Therapy
34 Adenovirus-Mediated Wild-Type
p53
Gene Transfer into...
... target gene and
reference gene (e.g., β-actin and GAPDH), then cloned to generate enough for
>10
9
assays. Internal standards for up to 1000 genes are quantified and mixed
together in a standardized ... reproducible, standardized, quantitative
measurement of data for many genes simultaneously. An internal standard CT is
prepared for each gene, cloned to generate enough...
... Mucins
Glycoprotein
Methods
and Protocols
Isolation of Large Gel-Forming Mucins 11
Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu-
cins were mixed with DNA and subjected ... mucin genes
(MUC1–MUC4, MUC5AC, MUC5B, and MUC6–MUC8) have been identified (for
reviews see refs. 1 and 2), with each gene showing expression in several tissues....