melanoma techniques and protocols

... E TM Melanoma Techniques and Protocols Edited by Brian J. Nickoloff, MD, PhD Molecular Diagnosis, Treatment, and Monitoring Melanoma Techniques and Protocols Molecular Diagnosis, Treatment, and ... Horikawa, T., Fujita, M., Middleton, M. H., and Morelli, J. G. (1996) Expression of E- and P-cadherin by melanoma cells decreases in progressive melanomas and following...

Ngày tải lên: 11/04/2014, 09:50

... Rogers Louise E. Donnelly Sampling Techniques and Analytical Protocols Human Airway Inflammation Edited by Duncan F. Rogers Louise E. Donnelly Sampling Techniques and Analytical Protocols M E T H O D S ... Sampling Techniques and Analytical Protocols Edited by: D. F. Rogers and L. E. Donnelly © Humana Press Inc., Totowa, NJ Airway Inflammation and Remodeling in Asthma 1...

Ngày tải lên: 11/04/2014, 09:46

... 10:55 AM20 Renal Disease Techniques and Protocols Edited by Michael S. Goligorsky, MD, PhD M E T H O D S I N M O L E C U L A R M E D I C I N E TM Renal Disease Techniques and Protocols Edited by Michael ... government and granting agencies. Standards of appropriate animal care and use are set forth in the Guide for the Care and Use of Laboratory Animals, commonly known as th...

Ngày tải lên: 11/04/2014, 10:28

... PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA PRESS Edited by Anthony P. Corfield The Mucins Glycoprotein Methods and Protocols Isolation ... acid func- tions and overall sequence similarity to prepro- von Willebrand factor. J. Biol. Chem. 269, 2440–2446. 5. Moniaux, N., Nollet, S., Porchet, N., De...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and...

Ngày tải lên: 08/10/2012, 10:25

... Methods and Protocols: The Mucins (Corfield, T. ed.), Humana, Totowa, NJ. 4. Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their ... sample volume, and superior wash- ing). For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samp...

Ngày tải lên: 08/10/2012, 10:25

... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so on can remove mucus ... to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195. 4. Bell, A. E., Allen, A., Morris, E. R., and Ro...

Ngày tải lên: 08/10/2012, 10:25

... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4. The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several ref...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......

Ngày tải lên: 08/10/2012, 10:25