immunocytochemical methods and protocols, 2nd
immunocytochemical methods and protocols, 2nd
... (Millipore).
Methods in Molecular Biology
Immunocytochemical
Methods and
Protocols
Second Edition
Edited by
Lorette C. Javois
HUMANA PRESS
HUMANA PRESS
Immunocytochemical
Methods and
Protocols
Second ... adsorbents.
Methods Enzym. 22, 273–286.
6. FPLC Ion Exchange and Chromatofocusing—Principles and Methods. (1985)
Pharmacia-LKB, Offsetcenter, Uppsala, Sweden.
7. Jaton, J...
immunocytochemical methods and protocols
... Filtration 21
4. England, S. and Seifter, S. (1990) Precipitation techmques. Methods Enzymol. 182,
285-296.
5. Jaton, J. C., Brandt, D. Ch., and Vassalli, P. (1979) The isolation and characteriza- ...
adsorbents. Methods Enzym. 22,273-286.
6. FPLC Ion Exchange and Chromatofocusmg-Principles and Methods (1985)
Pharmacia-LKB, Offsetcenter, Uppsala, Sweden.
7. Jaton, J C.,...
Glycoprotein Methods and Protocols - P1
... UV monitor and collect fractions using a fraction col-
lector and subject to the appropriate carbohydrate and antibody analyses.
3.6. Analysis of Mucins
Methods for the detection and analysis ... antibodies and
lectins or coating methods such as the glycan detection method and enzyme-linked
immunosorbent assays (ELISA). All these techniques have advantages and disadvan-
tage...
Glycoprotein Methods and Protocols - P2
... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,
NJ.
50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
Glycoprotein Methods and Protocols - P3
... Inc., Totowa, NJ
4
Detection and Quantitation of Mucins
Using Chemical, Lectin, and Antibody Methods
Michael A. McGuckin and David J. Thornton
1. Introduction
Detection and quantitation of mucins ... 1 and 2 (2,3) of this volume for the preparation of
secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a
discussion of methods for mucin separation....
Glycoprotein Methods and Protocols - P4
... poly-
L
-lysine for 5 min
and dried at room temperature.
3. Methods
3.1. Observation and Measurement
of Mucous Thickness on Unfixed Mucosal Sections (
see
Notes 1–4)
This is a rapid and simple method ... will be greatly
reduced and discontinuous.
7. Careful handling of the cryostat sections on the slide during fixing and staining is impor-
tant because excessive washing and so...
Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... NJ
6
Quantitation of Biosynthesis
and Secretion of Mucin Using Metabolic Labeling
Jan Dekker, B. Jan-Willem Van Klinken,
Hans A. Büller, and Alexandra W. C. Einerhand
1. Introduction
Most epithelial ... PhosphorImager.
4...
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77
7
Separation and Identification
of Mucins and Their Glycoforms
David J. Thornton, Nagma Khan, and John K. Sheehan
1. Introduction
This ......
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