immunocytochemical methods and protocols, 2nd
immunocytochemical methods and protocols, 2nd
... (Millipore). Methods in Molecular Biology Immunocytochemical Methods and Protocols Second Edition Edited by Lorette C. Javois HUMANA PRESS HUMANA PRESS Immunocytochemical Methods and Protocols Second ... adsorbents. Methods Enzym. 22, 273–286. 6. FPLC Ion Exchange and Chromatofocusing—Principles and Methods. (1985) Pharmacia-LKB, Offsetcenter, Uppsala, Sweden. 7. Jaton, J...
Ngày tải lên: 11/04/2014, 09:46
immunocytochemical methods and protocols
... Filtration 21 4. England, S. and Seifter, S. (1990) Precipitation techmques. Methods Enzymol. 182, 285-296. 5. Jaton, J. C., Brandt, D. Ch., and Vassalli, P. (1979) The isolation and characteriza- ... adsorbents. Methods Enzym. 22,273-286. 6. FPLC Ion Exchange and Chromatofocusmg-Principles and Methods (1985) Pharmacia-LKB, Offsetcenter, Uppsala, Sweden. 7. Jaton, J C.,...
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Glycoprotein Methods and Protocols - P1
... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the detection and analysis ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA). All these techniques have advantages and disadvan- tage...
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Glycoprotein Methods and Protocols - P2
... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...
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Glycoprotein Methods and Protocols - P3
... Inc., Totowa, NJ 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton 1. Introduction Detection and quantitation of mucins ... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation....
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Glycoprotein Methods and Protocols - P4
... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method ... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so...
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Glycoprotein Methods and Protocols - P5
... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4...
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Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......
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