hemostasis and thrombosis protocols
... assume an exten- Hemostasis 3 3 From: Methods in Molecular Medicine, Vol. 31: Hemostasis and Thrombosis Protocols Edited by: D. J. Perry and K. J. Pasi © Humana Press Inc., Totowa, NJ 1 Hemostasis Components ... 162, 156–159. Amplification of DNA and RNA by PCR 31 31 From: Methods in Molecular Medicine, Vol. 31: Hemostasis and Thrombosis Protocols Edited by: D. J. Perr...
Ngày tải lên: 11/04/2014, 09:45
... Trees and Control Protocols 2-103 The advantage of increasing the Query Interval and Query Response Interval is less overhead and bandwidth on the segment and less work for the routers and ... Cisco Systems, Inc. IP Multicast Distribution Trees and Control Protocols 2-105 In the example above, we have IGMPv2 router and hosts H1 and H3 and IGMPv1 host H2. The...
Ngày tải lên: 11/12/2013, 14:15
... lists and GNS filters to manage IPX traffic. 17 ○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○○ Networking and Desktop Protocols As you can see in Listing 7.15, the network filter stops network 2 and ... role of Cisco routers and operation of IPX in greater detail. IPX servers and printers send out SAPs (which are broadcast frames), and Cisco routers listen for the SAPs and install t...
Ngày tải lên: 21/12/2013, 19:15
basic dna and rna protocols
... bacteriophages and other viruses by agarose gel electrophoresis. J Vzrol 14, 1235-1244. CHAPTER 1 The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells Frank Merante, Sandeep ... Basrc DNA and RNA Protocols Edlted by A Harwood Humana Press Inc , Totowa, NJ 23 24 Smith and Murphy 3MM paper Hybrldisation Membrane Gel Transfer Buffer Reservoir \...
Ngày tải lên: 10/04/2014, 11:03
basic protein and peptide protocols
... alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively. 3. Plastic and glassware used in the assay should be absolutely clean and detergent-free. ... the reagent blank between 2 min and 1 h after mixing (see Note 5). The 100 pg standard should give an A595 value of about 0.4. The standard curve is not linear and the precise absorba...
Ngày tải lên: 10/04/2014, 11:03
antigen processing and presentation protocols
... SDS-PAGE analysis and proteasome activity assays, concentrate and store proteasomes according to Subheading 3.7. References 1. Monaco, J. J. and Nandi, D. (1995) Genetics of proteasomes and antigen ... 729–754. 2. Coux, O., Tanaka, K., and Goldberg, A. L. (1996) Structure and functions of the 20S and 26S proteasomes. Annu. Rev. Biochem. 65, 801–847. 3. Nandi, D., Marusina, K.,...
Ngày tải lên: 10/04/2014, 22:14
animal cell electroporation and electrofusion protocols
... commercial electroporation equipment, and many published and manufacturer-supplied protocols. Many of these pro- tocols are results of trial and error. These empirical protocols are valu- able guides ... population. With increasing molecular size and/ or charge, fewer and fewer pores should partici- pate, and this means that F,,, should decrease as the size and charge of...
Ngày tải lên: 11/04/2014, 09:32
gene isolation and mapping protocols
... Mural, R. J , and Uberbacher, E. C. (1994) User’s guide to GRAIL and GENQUEST (sequence analysis, gene assembly and sequence comparison systems) e-mail servers and XGRAIL (Version 1 2) and XGENQUEST ... Kruglyak, L. and Lander, E. S. (1995) Complete multipoint sib-pax analysis of quahtattve and quantitative traits. Am J, Hum Genet 57, 439-454 23. Hodge, S E. and Elston, E...
Ngày tải lên: 11/04/2014, 09:42
gene transfer and expression protocols
... 102 Shipley and Sheer tion into a particular region can be achieved by a random or a nonrandom approach. After random insertion of a vector carrying a selectable ... Filter-sterilize and store at -20°C. 5. Solution 3 (washing buffer): Same as solution 2, but without glucose. Autoclave and store at -2OOC. 12 A&in, Weinfeld, and Paterson and assuming that ... Transfer and...
Ngày tải lên: 11/04/2014, 09:42