hematologic malignancies, methods and techniques

... C I N E TM Edited by Guy B. Faguet, MD Hematologic Malignancies Methods and Techniques Edited by Guy B. Faguet, MD Hematologic Malignancies Methods and Techniques Humana Press Humana Press FISH ... 3 3 From: Methods in Molecular Medicine, vol. 55: Hematologic Malignancies: Methods and Techniques Edited by: G. B. Faguet © Humana Press Inc., Totowa, NJ 1 Cytogenetics A...

Ngày tải lên: 11/04/2014, 09:45

... radioisotope activity 423 17.6 Antigens and antibodies 425 x CONTENTS 17.7 Enzymatic-immunoassay (EIA) 426 17.8 Other immunoenzymatic techniques 429 17.9 Advantages and limitations of the ELISA test ... 455 19.3 Other ion selective electrodes 457 19.4 Slope and calculations 460 19.5 Applications 463 Problems 463 20 Voltammetric and coulometric methods 465 20.1 General principles 4...

Ngày tải lên: 01/04/2014, 10:59

... corpus providers (LPL and RALI) and the (RALI, LO- ILIA, ISSCO, IRMC and LIA). General coor- dination was handled by J. V~ronis (LPL); a discussion group was set up and moderated by Ph. Langlais ... parallel text alignment techniques. These techniques attempt to map various tex- tual units to their translation and have proven useful for a wide range of applicatious and...

Ngày tải lên: 08/03/2014, 05:21

... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA). All these techniques have advantages and disadvan- tages. Solution methods ... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the d...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory...

Ngày tải lên: 08/10/2012, 10:25

... Inc., Totowa, NJ 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton 1. Introduction Detection and quantitation of mucins ... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation....

Ngày tải lên: 08/10/2012, 10:25

... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method ... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolute ....

Ngày tải lên: 08/10/2012, 10:25