gene therapy of cancer, methods and protocols
gene therapy of cancer, methods and protocols
... CANCER GENE THERAPY A: Immunotherapy/Tumor Vaccination Cytokine-Transduced Tumor Cells 3 3 From: Methods in Molecular Medicine, Vol. 35: Gene Therapy: Methods and Protocols Edited by: W. Walther and ... Intratumoral Gene Transfer of the Cytosine Deaminase Gene for the Treatment of Breast Cancer Hardev S. Pandha and Nicholas R. Lemoine 523 C: Anti-Oncogene and Supp...
Ngày tải lên: 11/04/2014, 09:42
colorectal cancer, methods and protocols
... technique is time consuming and of low yield. Artifacts of cell culture include the selection of genetic alterations not present in primary tumors (1,2) and the alteration of gene expression as compared ... paraffin-embedded tumors) of each of both labeled DNAs (tumor and reference of the same sex), 20 µg of unlabeled Cot-1 DNA, 10 µL of 3 M Na acetate and 700 µL a...
Ngày tải lên: 10/04/2014, 22:22
ovarian cancer, methods and protocols
... mapping of tumor suppressor genes has made use of both cytogenetic and molecular analyses. One particularly useful approach has been loss of heterozygosity analysis in which patterns of loss of alleles ... tumors of low histological grade. 3.5.1. The p53 Gene The p53 gene on chromosome 17p13.1 is central to the control and regulation of DNA repair in cells. Deletions an...
Ngày tải lên: 11/04/2014, 10:00
prostate cancer, methods and protocols
... malignancy and the second highest cause of cancer death in Western soci- ety. The disease is very heterogeneous in terms of grade, genetics, ploidy, and oncogene/tumor suppressor gene expression, and ... prevalence and meaningfulness of multiple-case prostate cancer families. Because multiple-case families form the substrate for linkage analysis and gene hunting, and als...
Ngày tải lên: 11/04/2014, 10:11
renal cancer, methods and protocols
... MD Methods and Protocols Renal Cancer Edited by Jack H. Mydlo, MD Methods and Protocols RCC: Clinical Features and Management 21 the management of recurrent disease and the timing of referrals of ... site and number of metastatic deposits, completeness of resection, the performance status of the patient, and the disease-free interval from treatment of the pri...
Ngày tải lên: 11/04/2014, 10:28
nonviral vectors for gene therapy, methods and protocols
... synthesis of the peptides and a method of gene trans- fer using them. As is well known, a peptide is readily synthesized because of the development of an automatic peptide synthesis apparatus and reagents ... Formation of peptide–DNA complex and its transfer pathway into cell. M E T H O D S I N M O L E C U L A R M E D I C I N E TM Edited by Mark A. Findeis Nonviral Vectors f...
Ngày tải lên: 11/04/2014, 09:59
recombinant antibodies for cancer therapy, methods and protocols
... Therapy Methods and Protocols Methods and Protocols Generation of Antibody Molecules 21 49. Better, M., Chang, C. P., Robinson, R. R., and Horwitz, A. H. (1988) Escherichia coli secretion of an ... function of the solubility and stability of the native state of the protein, but of those of its folding intermediates in their particular environment (78,79). The d...
Ngày tải lên: 11/04/2014, 10:25
cancer cell culture, methods and protocols
... Cell Culture Methods and Protocols Edited by Simon P. Langdon M E T H O D S I N M O L E C U L A R M E D I C I N E TM Cancer Cell Culture Methods and Protocols Edited by Simon P. Langdon Basic Techniques of ... through a number of large cell banks. The largest of these are listed in Table 4 and in addition there are many other national collections that are often government...
Ngày tải lên: 10/04/2014, 11:11
cancer cell signaling methods and protocols
... peroxide generation in some cells (7), cause the aggregation of the TNF-receptor and activation of the JNK path- way (8) in the absence of any ligand. Thus, it appears that the oxidation-state of the ... His gene and a LacZ gene that have GAL4 promoters. Antimitogenic Activity of Tumor Suppression 3 1 Functional Analysis of the Antimitogenic Activity of Tumor Suppressors...
Ngày tải lên: 10/04/2014, 11:11
cancer cytogenetics, methods and protocols
... Biology TM Methods in Molecular Biology TM Edited by John Swansbury Cancer Cytogenetics VOLUME 220 Methods and Protocols Edited by John Swansbury Cancer Cytogenetics Methods and Protocols Cytogenetic ... probes for the ABL and BCR genes led to the discov- ery that approx 10% of translocations include deletion of part of one of these genes, usually the proximal part...
Ngày tải lên: 10/04/2014, 11:11