developmental biology protocols, volume i

... histological criteria are emphasized as a starting point for studying inductive interactions. Now, however, several specific antibodies are available for the identification of induced tis- sues, which can ... proteins that bind specific DNA sequences with relatively high affinity. This allows their direct isolation by affinity chromatography on columns bearing specific oligonucleotide tar- ge...

Ngày tải lên: 11/04/2014, 02:14

... and inhibin proteins. Activin A is a homodimer con- sisting of two inhibin β A chains; activin AB is a heterodimer of inhibin β A and β B chains; and activin B is a homodimer of two inhibin ... “inducing substances.” Activin is a dimeric protein and has been described in mammals as sharing subunits with a related dimeric protein—inhibin (Fig. 1). Inhibin is another peptide, which, in contr...

Ngày tải lên: 10/04/2014, 22:59

... are quite effective in entering target tissue and blocking specific gene expression (18,23–25). Several other options exist including delivery with cationic lipids and conjugation to fusigenic proteins ... treated with topically applied ODN (B and C) were stained with hematoxylin-eosin. Somitogenesis is absent in the somitic region of the embryo depicted in (B), consistent with absent epithel...

Ngày tải lên: 10/04/2014, 23:01

... are quite effective in entering target tissue and blocking specific gene expression (18,23–25). Several other options exist including delivery with cationic lipids and conjugation to fusigenic proteins ... treated with topically applied ODN (B and C) were stained with hematoxylin-eosin. Somitogenesis is absent in the somitic region of the embryo depicted in (B), consistent with absent epithel...

Ngày tải lên: 08/04/2014, 12:56

... owing to a neutralization of charge and an increase in rigidity that accompanies ethidium binding. 2. Materials 2.1. Plasmid DNA Negatively supercoiled plasmid DNAs can most readily be purified ... relaxation is reduced by inhibiting possi- bly contaminating nuclease with EDTA. 4. A typical sample volume in a well is 5 µL. This small volume often necessitates blot hybridization for topois...

Ngày tải lên: 10/04/2014, 23:16

... BigInteger add(BigInteger other) • BigInteger subtract(BigInteger other) • BigInteger multiply(BigInteger other) • BigInteger divide(BigInteger other) • BigInteger mod(BigInteger other) ... java.math.BigDecimal • BigDecimal add(BigDecimal other) • BigDecimal subtract(BigDecimal other) • BigDecimal multiply(BigDecimal other) • BigDecimal divide(BigDecimal other, int roundin...

Ngày tải lên: 31/07/2013, 09:23

... this timer the expiration timer, or timeout . Cisco's IOS calls it the invalid timer. The expiration timer is initialized to 180 s econds whenever a new route is established and is ... static routing is that it is hard to administer. This criticism may be true of medium to large topologies with many alternative routes, but it is certainly not true of small inter networks with few...

Ngày tải lên: 10/12/2013, 16:15

... value equals x . • BigDecimal add(BigDecimal other) • BigDecimal subtract(BigDecimal other) • BigDecimal multiply(BigDecimal other) • BigDecimal divide(BigDecimal other, RoundingMode mode) 5.0 returns ... other) • BigInteger divide(BigInteger other) • BigInteger mod(BigInteger other) returns the sum, difference, product, quotient, and remainder of this big integer and other . • int compare...

Ngày tải lên: 24/12/2013, 12:16

... laughing, ‘and I agree with you entirely, that it will be a much better thing. Invite him to dinner, Emma, and help him to the best of the fish and the chicken, but leave him to chuse his own wife. ... joining their hands to-day, he looked so very much as if he would like to have the same kind office done for him! I think very well of Mr. Elton, and this is the only way I have of...

Ngày tải lên: 24/12/2013, 13:16

... and to live according to the wishes of his own friendly and social disposition. It was now some time since Miss Taylor had begun to influence his schemes; but as it was not the tyrannic influence ... satisfied an active, cheerful mind and social temper by entering into the militia of his county, then embodied. Captain Weston was a general favourite; and when the chances of his military...

Ngày tải lên: 24/12/2013, 13:16