bacterial artificial chromosomes, volume 1

... 10 µL. 13 . Incubate at 37°C for 15 min. 14 . Add 1 U of CIP (1 µL) and incubate at 37°C for 1 h. 15 . Inactivate the enzymes and recover the DNA by following steps 6 10 . 16 . Dissolve in 15 0 µL ... casting stand, platform ( 21 × 14 cm), and gel-casting stand (cat. no. 17 0-3704; Bio-Rad). 10 . Plastic seal (TR100, Therma Seal Plate Film 2.0 PP, PK /10 0; Marsh Biomedical)....

Ngày tải lên: 11/04/2014, 00:36

... 7.9), 10 % glycerol, 0 .1% Triton X -10 0, 10 mM β-mercaptoethanol. 10 . Buffer C: 2 M urea, 1 M NaCl, 20 mM HEPES (pH 7.9), 10 % glycerol, 0 .1% Triton X -10 0, 10 mM β-mercaptoethanol. 11 . Buffer D: 50 mM ... 32 P. 11 . pSPL3 VV oligo sequence: CGA CCC AGC A|AC CTG GAG AT. 12 . pSPL3 10 21 oligo sequence: AGC TCG AGC GGC CGC TGC AG. 13 . pSPL3 11 71 oligo sequence: AGA CC...

Ngày tải lên: 11/04/2014, 00:42

... oncogenes such as MYCN, NAG and DDX1 were located in these regions. The less frequent region of gain localized to 17 q23.2, 17 q23.3 -17 q24 .1, 17 q24 .1- 17q24.2, 17 q25.2 -17 q25.3 was detected in about ... gH/gL/pUL (12 8 ,13 0 ,13 1A) content. UL128, UL130, and UL131A are required for replication of HCMV in HUVECs (Hahn, Revello et al. 2004). EC tropism for HCMV is highly depend...

Ngày tải lên: 21/02/2014, 23:20

... proportion (4, 5 in the argument 3–7; 12 , 13 in the argument 10 15 ; 16 , 18 in the argument 15 19 ). In Steps 3–7, both ratios are substituted. In 10 15 and 15 19 , only one 90 on the sphere and the ... Elements V .18 . 19 3 Interlude, recalling Elements XII .14 . 19 4 Elements V.9. 19 5 SC I .15 . 19 6 Elements VI.4. 88 on the sphere and the cylinder i The answer may be the fol...

Ngày tải lên: 21/09/2012, 11:00

... pirated, call 1- 800-PIRATES or 1- 8 01- 8 61- 710 1. The Novell Guide to Network + (Volume 1) COURSE 14 00 The Novell ® Guide to Network + TM Volume 1 10 0-004484-0 01 Revision 1. 0 The ... Novell-certified instructor. IG 1- 113 If you think this document was pirated, call 1- 800-PIRATES or 1- 8 01- 8 61- 710 1. Cable Media (continued) d 1- 15 (Figure...

Ngày tải lên: 22/10/2013, 16:15

... Configuration Manual 4-35 C79000-G8276-C139- 01 Part of a program to explain pointers { int number1; int *pointer1; number1 = 12 3; pointer1 = &number1; } This example is the best way of explaining ... a,b,c; int result1,result2,result3,result4,result5; a=3; // binary 011 b=5; // binary 10 1 result1=a&b; // AND result2=a|b; // inclusive OR result3=a^b; // exclusive OR result4=a&...

Ngày tải lên: 02/11/2013, 14:36

... budget on improving public transportation. TOEFL Essay Collection Volume 1 Collected by elearningclub.net Page 21 of 59 Topic 20: It is better for children to grow up in the countryside ... Topic 21: In general, people are living longer now. Discuss the causes of this phenomenon. Use specific reasons and details to develop your essay. Score: 6.0 TOEFL Essay Collection V...

Ngày tải lên: 20/11/2013, 02:05

... ID (19 2 .16 8 .1. 67) (Process ID 10 ) Router Link States (Area 1) Link ID ADV Router Age Seq# Checksum Link count 19 2 .16 8 .1. 67 19 2 .16 8 .1. 67 48 0x80000008 0xB 112 2 19 2 .16 8.2 .13 0 19 2 .16 8.2 .13 0 212 0x80000006 ... reserved. BSCI v3.0—3 -11 debug ip ospf packet R1#debug ip ospf packet OSPF packet debugging is on R1# *Feb 16 11 :03: 51. 206: OSPF: rcv. v:2 t :1 l:4...

Ngày tải lên: 13/12/2013, 10:15

... expressed. (a)  m n =1 1 n k , for k =1, 2, 3 (b)  m n =1 6n+3 4n 4 +8n 3 +8n 2 +4n+3 [Abra 71] (c)  m 1 n =1 n 2 −2n 1 n 2 (n +1) 2 2 n (d)  m n =1 n 2 4 n (n +1) (n+2) (e)  m 1 n=0 2 n n +1 [Man93] (f)  m n=4 4 (1 n)(n 2 −2n 1) n 2 (n +1) 2 (n−2) 2 (n−3) 2 [Man93] (g)  m n =1 (n 4 14 n 2 −24n−9)2 n n 2 (n +1) 2 (n+2) 2 (n+3) 2 [Man93] (h) m  n =1  n 1 j =1 j 3  n...

Ngày tải lên: 20/12/2013, 19:15