dna topoisomerase protocols, volume i

... Shape Circular plasmid DNA can exist in a number of different topological confor- mations. Superhelical circular DNA (form I) , nicked circular DNA (form II), and linear DNA (form III) of identical ... of supercoiling have similar mobilities, and individual topoisomers are not resolved. The electrophoretic mobility of a DNA ring can be altered without changing its linking number. T...

Ngày tải lên: 10/04/2014, 23:16

... depending on the total salt con- centration), 0.5–1 µg kDNA, topoisomerase II inhibitors if required, and various concentrations of topoisomerase II-containing extracts or purified topoisomerase II. ... topoisomerase II activity during the early stages of enzyme purification can mask the true elution profile of topoisomerase II isoforms. Fig. 2. Decatenation of kDNA by topoisomeras...

Ngày tải lên: 11/04/2014, 00:21

... proteins that bind specific DNA sequences with relatively high affinity. This allows their direct isolation by affinity chromatography on columns bearing specific oligonucleotide tar- get sites ... histological criteria are emphasized as a starting point for studying inductive interactions. Now, however, several specific antibodies are available for the identification of induced tis- sues,...

Ngày tải lên: 11/04/2014, 02:14

... BigInteger add(BigInteger other) • BigInteger subtract(BigInteger other) • BigInteger multiply(BigInteger other) • BigInteger divide(BigInteger other) • BigInteger mod(BigInteger other) ... java.math.BigDecimal • BigDecimal add(BigDecimal other) • BigDecimal subtract(BigDecimal other) • BigDecimal multiply(BigDecimal other) • BigDecimal divide(BigDecimal other, int roundin...

Ngày tải lên: 31/07/2013, 09:23

... this timer the expiration timer, or timeout . Cisco's IOS calls it the invalid timer. The expiration timer is initialized to 180 s econds whenever a new route is established and is ... static routing is that it is hard to administer. This criticism may be true of medium to large topologies with many alternative routes, but it is certainly not true of small inter networks with few...

Ngày tải lên: 10/12/2013, 16:15

... value equals x . • BigDecimal add(BigDecimal other) • BigDecimal subtract(BigDecimal other) • BigDecimal multiply(BigDecimal other) • BigDecimal divide(BigDecimal other, RoundingMode mode) 5.0 returns ... other) • BigInteger divide(BigInteger other) • BigInteger mod(BigInteger other) returns the sum, difference, product, quotient, and remainder of this big integer and other . • int compare...

Ngày tải lên: 24/12/2013, 12:16

... laughing, ‘and I agree with you entirely, that it will be a much better thing. Invite him to dinner, Emma, and help him to the best of the fish and the chicken, but leave him to chuse his own wife. ... joining their hands to-day, he looked so very much as if he would like to have the same kind office done for him! I think very well of Mr. Elton, and this is the only way I have of...

Ngày tải lên: 24/12/2013, 13:16

... and to live according to the wishes of his own friendly and social disposition. It was now some time since Miss Taylor had begun to influence his schemes; but as it was not the tyrannic influence ... satisfied an active, cheerful mind and social temper by entering into the militia of his county, then embodied. Captain Weston was a general favourite; and when the chances of his military...

Ngày tải lên: 24/12/2013, 13:16

... manners. It would be an interesting, and certainly a very kind undertaking; highly becoming her own situation in life, her leisure, and powers. She was so busy in admiring those soft blue eyes, in ... Austen Volume I Chapter III Mr. Woodhouse was fond of society in his own way. He liked very much to have his friends come and see him; and from various united causes, from his lon...

Ngày tải lên: 24/12/2013, 13:16

... Austen Volume I Chapter IV Harriet Smith’s intimacy at Hartfield was soon a settled thing. Quick and decided in her ways, Emma lost no time in inviting, encouraging, and telling her to ... gained in Harriet’s inclination. Harriet was not insensible of manner; she had voluntarily noticed her father’s gentleness with admiration as well as wonder. Mr. Martin looked as if he did ... h...

Ngày tải lên: 24/12/2013, 13:16