e coli, shiga toxin methods and protocols

... to cloned stx 1 and stx 2 genes and their respective nucleotide sequences enabled the development of DNA and oligonucleotide probes for the detection of STEC (reviewed in ref. 5). The introduction ... the LEE (locus of enterocyte effacement) pathogenicity island (2,5,7). However, spo- radic cases of HUS have been associated with over 100 different LEE-positive and LEE-negative STEC...

Ngày tải lên: 23/03/2014, 07:20

... rea- sons, STEC detection methods need to be very sensitive and require minimal specimen volumes. Shiga toxigenic E. coli diagnostic methods are based on the detection of the presence of either Stx ... the subtractive hybridization method. Black lines represent the digested tester DNA and grey lines represent the digested driver DNA. The two different adaptor sequences are given in...

Ngày tải lên: 10/04/2014, 23:03

... be pierced with a needle to equilibrate the pressure, and the DFP should be transferred using a syringe and needle. The contents of the vial should be placed directly into the correct volume ... adhere to the membrane may be lost and the linear range of the technique may be limited. “Coating methods such as ELISA are prone to artefacts if samples are concentrated, and care must be ta...

Ngày tải lên: 08/10/2012, 10:25

... site has been selected for study, it is desirable that the results be presented in a standardized manner. The size of the area to be assessed may be predetermined, but this is more likely to be ... blood vessels. Whereas at least some positive structures are not guar- anteed in every section, sections from positive tissue that been fixed and processed in the same manner as the test samples...

Ngày tải lên: 08/10/2012, 10:25

... although effective against the protein precursors, were of little use for mature mucins. Nevertheless, chemical and/ or enzymatic deglycosylation techniques can be used to increase the effectiveness ... homology and therefore cross- reactivity (5). Many of the early MUC-specific probes were generated against sequences underlying the highly glycosylated tandem repeat regions of the molecule...

Ngày tải lên: 08/10/2012, 10:25

... adherent to the mucosal surface in vivo is determined by a balance between the rate of secretion by the underlying epithelium and the rate of erosion by mechanical shear associated with the digestive ... adherent mucous thickness in situ were based on (1) differences in refractive index between the mucous layer and the mucosal surface, measured by a slit lamp and pachymeter (16); (2) th...

Ngày tải lên: 08/10/2012, 10:25