e coli, shiga toxin methods and protocols

... to cloned stx 1 and stx 2 genes and their respective nucleotide sequences enabled the development of DNA and oligonucleotide probes for the detection of STEC (reviewed in ref. 5). The introduction ... the LEE (locus of enterocyte effacement) pathogenicity island (2,5,7). However, spo- radic cases of HUS have been associated with over 100 different LEE-positive and LEE-negative STEC...

Ngày tải lên: 23/03/2014, 07:20

... rea- sons, STEC detection methods need to be very sensitive and require minimal specimen volumes. Shiga toxigenic E. coli diagnostic methods are based on the detection of the presence of either Stx ... the subtractive hybridization method. Black lines represent the digested tester DNA and grey lines represent the digested driver DNA. The two different adaptor sequences are given in...

Ngày tải lên: 10/04/2014, 23:03

... site has been selected for study, it is desirable that the results be presented in a standardized manner. The size of the area to be assessed may be predetermined, but this is more likely to be ... blood vessels. Whereas at least some positive structures are not guar- anteed in every section, sections from positive tissue that been fixed and processed in the same manner as the test samples...

Ngày tải lên: 08/10/2012, 10:25

... although effective against the protein precursors, were of little use for mature mucins. Nevertheless, chemical and/ or enzymatic deglycosylation techniques can be used to increase the effectiveness ... homology and therefore cross- reactivity (5). Many of the early MUC-specific probes were generated against sequences underlying the highly glycosylated tandem repeat regions of the molecule...

Ngày tải lên: 08/10/2012, 10:25