gene expression profiling, methods and protocols

... target gene and reference gene (e.g., β-actin and GAPDH), then cloned to generate enough for >10 9 assays. Internal standards for up to 1000 genes are quantified and mixed together in a standardized ... Internal standard Standard curve of CT for each gene reference sample in an SMIS compared to test sample 2 Edited by Richard A. Shimkets Gene Expression Profiling Methods...

Ngày tải lên: 10/04/2014, 22:32

... predetermined. Methods and Protocols Methods and Protocols Gene Therapy of Cancer M E T H O D S I N M O L E C U L A R M E D I C I N E TM Humana Press Edited by Wolfgang Walther Ulrike Stein Gene Therapy of ... CANCER GENE THERAPY A: Immunotherapy/Tumor Vaccination Cytokine-Transduced Tumor Cells 3 3 From: Methods in Molecular Medicine, Vol. 35: Gene Therapy: Methods a...

Ngày tải lên: 11/04/2014, 09:42

... peptide–DNA complex and its transfer pathway into cell. M E T H O D S I N M O L E C U L A R M E D I C I N E TM Edited by Mark A. Findeis Nonviral Vectors for Gene Therapy Methods and Protocols Humana ... Protocols Humana Press Humana Press Edited by Mark A. Findeis Nonviral Vectors for Gene Therapy Methods and Protocols 20 Niidome and Aoyagi internalization of Gal 4 –4 6...

Ngày tải lên: 11/04/2014, 09:59

... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties,...

Ngày tải lên: 08/10/2012, 10:25

... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... amount of radioa...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

Ngày tải lên: 08/10/2012, 10:25