vectors in gene therapy

... deleting retroviral genes and adding gene( s) of interest. Vectors can be produced in pack- aging cell lines that express packaging genes. The major advantage of retroviral vectors is the precise integration ... AAV in gene therapy is the ability to transfer genetic information into nondividing cells in vivo. In addition, expression has been maintain for up to one year. Furth...

Ngày tải lên: 10/04/2014, 22:11

. concentration in solution is determined from UV-absorbance of Trp at 280 nm in a buffer containing 6 M Gu • HCl (¡ = 5500). 3.4.2. Gene Transfer Using Galactose-Modified Peptide into Hepatoma Cell Line,. targeted gene transfer has also been realized by modification of the gene carriers using cell-targeting ligands such as asialoorosomucoid, transferrin, insulin, or galactose. Recently, novel gen...

Ngày tải lên: 11/04/2014, 09:59

. illustrated in Fig. 5. 1.4. Inserting Transgenes into Replication-Defective Vectors Insertion of transgenes into the replication-defective HSV vectors is achieved by homologous recombination in eukaryotic. particles that contain very few viral gene sequences. In this instance, the desired transgene cassette is placed in a plasmid containing the viral genomic packaging/cleavage signals, in a...

Ngày tải lên: 11/04/2014, 10:39

... proviral form of the vector is shown in Fig. 1. The packaging system contains the self-inactivating transducing constructs (sin) obtained after a 400-bp deletion including the enhancer and promoter from ... of co-incubated, untransduced cells. Len- tiviral vectors have been shown to effectively transduce genes into a range of both dividing and nondividing cell types, including neurons, re...

Ngày tải lên: 24/03/2014, 04:21

... recombinant BMP6 adenoviral vectors in immunocompetent animals. 1. INTRODUCTION Gene therapy provides a novel method to repair damaged bone by using bone morphogenetic protein (BMP). BMP gene ... diseases in the human clinical setting. Besides the aforementioned major findings of this experiment, accurate gene sequencing is also a critical factor in the determination of...

Ngày tải lên: 31/10/2012, 17:08

... distinctions in mind we can raise some additional ethics issues regarding gene therapy from a social point of view. Is there anything intrinsically morally objectionable about gene therapy in ... that would support a presumption in favor of continuing the development of germline genetic engineering. That is, we have argued that germline genetic engineering is not intrinsically m...

Ngày tải lên: 10/04/2014, 22:10

... SJ, Baker AH, Angelini GD. Gene transfer of tissue inhibitor of metalloproteinase- 2 inhibits metalloproteinase activity and neointima formation in human saphenous veins. Gene Therapy 5:1552–1560, ... cardiovascular gene therapy. Hum Gene Therapy 10:3031–3044, 1999. Mann MJ, Gibbons GH, Tsao PS. Cell cycle inhibition preserves endothelial function in genetically engineered...

Ngày tải lên: 10/04/2014, 22:11

... amplifying the local in ammatory 252 GENE THERAPY IN THE TREATMENT OF CANCER response. Clinical trials using this approach are now in progress in melanoma patients. Cytokines Cytokines are proteins ... CML AML1/MTG8 AML now in development. Such inhibition results not only in growth inhibition in vitro but also results in growth inhibition of tumors in animal models of car...

Ngày tải lên: 10/04/2014, 22:11